Institut für Chemie und Bioanalytik
Dauerhafte URI für die Sammlunghttps://irf.fhnw.ch/handle/11654/24
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Publikation Scaling down the heat transfer in multipurpose plants(26.06.2024) Zogg, Andreas04B - Beitrag KonferenzschriftPublikation Kontinuierliche Ethoxylierung(25.04.2024) Zogg, Andreas; Zahnd, This; Brönnimann, BenediktModellbasiertes Sicherheitskonzept für die Entwicklung einer kontinuierlichen Ethoxylierung. Modellvalidierung mittels kontinuierlichem Kalorimeter innerhalb einer Glove-Box.06 - PräsentationPublikation Development of a novel measurement setup to study and predict electrostatic discharges in agitated glass‐lined vessels(Wiley, 12.02.2024) Brönnimann, Benedikt; Egli‐Tedesco, Daniel; Schwenzfeuer, Klaus; Zogg, AndreasTwo glass lined reactors in a launch platform facility operated by Syngenta have been damaged during the crystallization of an organic compound due to electrostatic discharges. The goal of this work was to design and commission a novel setup to measure charges and currents generated by this slurry in a laboratory‐scale reactor. An improved and more sophisticated setup was then proposed for possible implementation in Syngenta's own laboratories. With this novel setup, the electrostatic charging of stirred suspensions involving nonconductive solvents could be accurately measured in the context of a case study that involved the suspension that led to liner damages in the production facilities of Syngenta.01A - Beitrag in wissenschaftlicher ZeitschriftPublikation Prozessoptimierung von 4 Stufen zur Herstellung eines Pharma-Zwischenprodukts(Hochschule für Life Sciences FHNW, 2024) Güner, Merve; Zogg, Andreas11 - Studentische ArbeitPublikation Development of a tool for the automated visualization of batch recipes(Hochschule für Life Sciences FHNW, 2024) Gentner, Jannick; Zogg, Andreas; Bachem AG11 - Studentische ArbeitPublikation Modeling-based approach towards quality by design for a telescoped process(Schweizerische Chemische Gesellschaft, 2024) Zahnd, This; Kandziora, Maja; Levis, Michael K.; Zogg, AndreasA telescoped, two-step synthesis was investigated by applying Quality by Design principles. A kinetic model consisting of 12 individual reactions was successfully established to describe the synthesis and side reactions. The resulting model predicts the effects of changes in process parameters on total yield and quality. Contour plots were created by varying process parameters and displaying the model predicted process response. The areas in which the process response fulfils predetermined quality requirements are called design spaces. New ranges for process parameters were explored within these design spaces. New conditions were found that increased the robustness of the process and allowed for a considerable reduction of the used amounts of a reagent. Further optimizations, based on the newly generated knowledge, are expected. Improvements can either be direct process improvements or enhancements to control strategies. The developed strategies can also be applied to other processes, enhancing upcoming and preexisting research and development efforts.01A - Beitrag in wissenschaftlicher ZeitschriftPublikation High-throughput silica nanoparticle detection for quality control of complex early life nutrition food matrices(American Chemical Society, 2024) Maffeis, Viviana; Otter, Andrea; Düsterloh, André; Kind, Lucy; Palivan, Cornelia; Saxer, SinaThe addition of nanomaterials to improve product properties has become a matter of course for many commodities: e.g., detergents, cosmetics, and food products. While this practice improves product characteristics, the increasing exposure and potential impact of nanomaterials (<100 nm) raise concerns regarding both the human body and the environment. Special attention should be taken for vulnerable individuals such as those who are ill, elder, or newborns. But detecting and quantifying nanoparticles in complex food matrices like early life nutrition (ELN) poses a significant challenge due to the presence of additional particles, emulsion-droplets, or micelles. There is a pressing demand for standardized protocols for nanoparticle quantification and the specification of “nanoparticle-free” formulations. To address this, silica nanoparticles (SiNPs), commonly used as anticaking agents (AA) in processed food, were employed as a model system to establish characterization methods with different levels of accuracy and sensitivity versus speed, sample handling, and automatization. Different acid treatments were applied for sample digestion, followed by size exclusion chromatography. Morphology, size, and number of NPs were measured by transmission electron microscopy, and the amount of Si was determined by microwave plasma atomic emission spectrometry. This successfully enabled distinguishing SiNP content in ELN food formulations with 2–4% AA from AA-free formulations and sorting SiNPs with diameters of 20, 50, and 80 nm. Moreover, the study revealed the significant influence of the ELN matrix on sample preparation, separation, and characterization steps, necessitating method adaptations compared to the reference (SiNP in water). In the future, we expect these methods to be implemented in standard quality control of formulation processes, which demand high-throughput analysis and automated evaluation.01A - Beitrag in wissenschaftlicher ZeitschriftPublikation Electrospun decellularized extracellular matrix scaffolds promote the regeneration of injured neurons(Elsevier, 09/2023) Mungenast, Lena; Nieminen, Ronya; Gaiser, Carine; Faia-Torres, Ana Bela; Rühe, Jürgen; Suter-Dick, Laura01A - Beitrag in wissenschaftlicher ZeitschriftPublikation The effect of the Pd precursors on the shape of hollow Ag–Pd alloy nanoparticles using Ag nanocubes as seeds(American Chemical Society, 28.07.2023) Wen, Xin; Nazemi, Amir; da Silva, Robson Rosa; Moth-Poulsen, Kasper01A - Beitrag in wissenschaftlicher ZeitschriftPublikation New scale-up technologies for multipurpose pharmaceutical production plants. Use case of a heterogeneous hydrogenation process(American Chemical Society, 06.07.2023) Furrer, Thierry; Levis, Michael; Berger, Bernhard; Kandziora, Maja; Zogg, Andreas01A - Beitrag in wissenschaftlicher ZeitschriftPublikation Development and validation of a liquid chromatography-triple quadrupole mass spectrometry method for the determination of isopeptide ε-(γ-glutamyl) lysine in human urine as biomarker for transglutaminase 2 cross-linked proteins(Elsevier, 21.06.2023) Dejager, Lien; Jairaj, Mark; Jones, Kieran; Johnson, Timothy; Dudal, Sherri; Dudal, Yves; Shahgaldian, Patrick; Correro, Rita; Qu, Jun; An, Bo; Lucey, Richard; Szarka, Szabolcs; Wheller, Robert; Pruna, Alina; Kettell, Sarah; Pitt, Andrew; Cutler, Paul01A - Beitrag in wissenschaftlicher ZeitschriftPublikation The monomeric archaeal primase from Nanoarchaeum equitans harbours the features of heterodimeric archaeoeukaryotic primases and primes sequence-specifically(Oxford University Press, 09.06.2023) Schneider, Andy; Bergsch, Jan; Lipps, GeorgThe marine thermophilic archaeon Nanoarchaeum equitans possesses a monomeric primase encompassing the conserved domains of the small catalytic and the large regulatory subunits of archaeoeukaryotic heterodimeric primases in one protein chain. The recombinant protein primes on templates containing a triplet with a central thymidine, thus displaying a pronounced sequence specificity typically observed with bacterial type primases only. The N. equitans primase (NEQ395) is a highly active primase enzyme synthesizing short RNA primers. Termination occurs preferentially at about nine nucleotides, as determined by HPLC analysis and confirmed with mass spectrometry. Possibly, the compact monomeric primase NEQ395 represents the minimal archaeoeukaryotic primase and could serve as a functional and structural model of the heterodimeric archaeoeukaryotic primases, whose study is hindered by engagement in protein assemblies and rather low activity.01A - Beitrag in wissenschaftlicher ZeitschriftPublikation HLA antibody affinity determination. From HLA‐specific monoclonal antibodies to donor HLA specific antibodies (DSA) in patient serum(Wiley, 16.05.2023) Hug, Melanie N.; Keller, Sabrina; Marty, Talea; Gygax, Daniel; Meinel, Dominik; Spies, Peter; Handschin, Joëlle; Kleiser, Marc; Vazquez, Noemi; Linnik, Janina; Buchli, Rico; Claas, Frans; Heidt, Sebastiaan; Kramer, Cynthia S. M.; Bezstarosti, Suzanne; Lee, Jar‐How; Schaub, Stefan; Hönger, GideonOrgans transplanted across donor‐specific HLA antibodies (DSA) are associated with a variety of clinical outcomes, including a high risk of acute kidney graft rejection. Unfortunately, the currently available assays to determine DSA characteristics are insufficient to clearly discriminate between potentially harmless and harmful DSA. To further explore the hazard potential of DSA, their concentration and binding strength to their natural target, using soluble HLA, may be informative. There are currently a number of biophysical technologies available that allow the assessment of antibody binding strength. However, these methods require prior knowledge of antibody concentrations. Our objective within this study was to develop a novel approach that combines the determination of DSA‐affinity as well as DSA‐concentration for patient sample evaluation within one assay. We initially tested the reproducibility of previously reported affinities of human HLA‐specific monoclonal antibodies and assessed the technology‐specific precision of the obtained results on multiple platforms, including surface plasmon resonance (SPR), bio‐layer interferometry (BLI), Luminex (single antigen beads; SAB), and flow‐induced dispersion analysis (FIDA). While the first three (solid‐phase) technologies revealed comparable high binding‐strengths, suggesting measurement of avidity, the latter (in‐solution) approach revealed slightly lower binding‐strengths, presumably indicating measurement of affinity. We believe that our newly developed in‐solution FIDA‐assay is particularly suitable to provide useful clinical information by not just measuring DSA‐affinities in patient serum samples but simultaneously delivering a particular DSA‐concentration. Here, we investigated DSA from 20 pre‐transplant patients, all of whom showed negative CDC‐crossmatch results with donor cells and SAB signals ranging between 571 and 14899 mean fluorescence intensity (MFI). DSA‐concentrations were found in the range between 11.2 and 1223 nM (median 81.1 nM), and their measured affinities fall between 0.055 and 24.7 nM (median 5.34 nM; 449‐fold difference). In 13 of 20 sera (65%), DSA accounted for more than 0.1% of total serum antibodies, and 4/20 sera (20%) revealed a proportion of DSA even higher than 1%. To conclude, this study strengthens the presumption that pre‐transplant patient DSA consists of various concentrations and different net affinities. Validation of these results in a larger patient cohort with clinical outcomes will be essential in a further step to assess the clinical relevance of DSA‐concentration and DSA‐affinity.01A - Beitrag in wissenschaftlicher ZeitschriftPublikation Allosteric targeting resolves limitations of earlier LFA-1 directed modalities(Elsevier, 05/2023) Mancuso, Riccardo V.; Schneider, Gisbert; Hürzeler Müller, Marianne; Gut, Martin; Zurflüh, Jonas; Breitenstein, Werner; Bouitbir, Jamal; Reisen, Felix; Atz, Kenneth; Ehrhardt, Claus; Duthaler, Urs; Gygax, Daniel; Schmidt, Albrecht G.; Krähenbühl, Stephan; Weitz-Schmidt, Gabriele01A - Beitrag in wissenschaftlicher ZeitschriftPublikation Die Chemie Pilotanlage der FHNW Muttenz(25.04.2023) Zogg, Andreas; Asprion, JonasDer Verein Miniplant 4.0 entwickelt im Process Technology Center an der FHNW neuartige Chemie-Pilotanlagen im Miniplant-Massstab. Der zentrale Scale-Down-Reaktor dient der präzisen Prozessentwicklung im Rührkessel. Insbesondere werden darin die lokalen thermischen Verhältnisse des Produktionsreaktors durch den Einsatz eines speziell designten Wärmetauschers exakt nachgebildet. Damit wird den Studenten und lokalen Unternehmen ein neuartiges Entwicklungswerkzeug zur Verfügung gestellt, welches einen schnelleren und präziseren Scale-Up von Produktionsverfahren direkt aus dem Labormassstab in den Produktionsreaktor erlaubt. Herr Prof. Dr. Andreas Zogg von der FHNW in Muttenz zeigt Ihnen den aktuellen Status der Anlage und die vielseitigen Schulungs- und Forschungsmöglichkeiten. Unterstützt wird er durch Herrn Dr. Jonas Asprion von der Firma Tool-Temp AG, welche die Kommerzialisierung der Temperierlösungen für Reaktormantel und Wärmetauscher anstrebt.06 - PräsentationPublikation CanIsoNet: a database to study the functional impact of isoform switching events in diseases(Oxford University Press, 17.04.2023) Karakulak, Tülay; Szklarczyk, Damian; Saylan, Cemil Can; Moch, Holger; von Mering, Christian; Kahraman, Abdullah; Ouangraoua, AidaMotivation: Alternative splicing, as an essential regulatory mechanism in normal mammalian cells, is frequently disturbed in cancer and other diseases. Switches in the expression of most dominant alternative isoforms can alter protein interaction networks of associated genes giving rise to disease and disease progression. Here, we present CanIsoNet, a database to view, browse and search isoform switching events in diseases. CanIsoNet is the first webserver that incorporates isoform expression data with STRING interaction networks and ClinVar annotations to predict the pathogenic impact of isoform switching events in various diseases. Results: Data in CanIsoNet can be browsed by disease or searched by genes or isoforms in annotation-rich data tables. Various annotations for 11 811 isoforms and 14 357 unique isoform switching events across 31 different disease types are available. The network density score for each disease-specific isoform, PFAM domain IDs of disrupted interactions, domain structure visualization of transcripts and expression data of switched isoforms for each sample is given. Additionally, the genes annotated in ClinVar are highlighted in interactive interaction networks. Availability and implementation: CanIsoNet is freely available at https://www.caniso.net. The source codes can be found under a Creative Common License at https://github.com/kahramanlab/CanIsoNet_Web.01A - Beitrag in wissenschaftlicher ZeitschriftPublikation In vitro to in vivo extrapolation and high-content imaging for simultaneous characterization of chemically induced liver steatosis and markers of hepatotoxicity(Springer, 12.04.2023) Müller, Fabrice A.; Stamou, Marianna; Englert, Felix H.; Frenzel, Ole; Diedrich, Sabine; Suter-Dick, Laura; Wambaugh, John F.; Sturla, Shana J.Chemically induced steatosis is characterized by lipid accumulation associated with mitochondrial dysfunction, oxidative stress and nucleus distortion. New approach methods integrating in vitro and in silico models are needed to identify chemicals that may induce these cellular events as potential risk factors for steatosis and associated hepatotoxicity. In this study we used high-content imaging for the simultaneous quantification of four cellular markers as sentinels for hepatotoxicity and steatosis in chemically exposed human liver cells in vitro. Furthermore, we evaluated the results with a computational model for the extrapolation of human oral equivalent doses (OED). First, we tested 16 reference chemicals with known capacities to induce cellular alterations in nuclear morphology, lipid accumulation, mitochondrial membrane potential and oxidative stress. Then, using physiologically based pharmacokinetic modeling and reverse dosimetry, OEDs were extrapolated from data of any stimulated individual sentinel response. The extrapolated OEDs were confirmed to be within biologically relevant exposure ranges for the reference chemicals. Next, we tested 14 chemicals found in food, selected from thousands of putative chemicals on the basis of structure-based prediction for nuclear receptor activation. Amongst these, orotic acid had an extrapolated OED overlapping with realistic exposure ranges. Thus, we were able to characterize known steatosis-inducing chemicals as well as data-scarce food-related chemicals, amongst which we confirmed orotic acid to induce hepatotoxicity. This strategy addresses needs of next generation risk assessment and can be used as a first chemical prioritization hazard screening step in a tiered approach to identify chemical risk factors for steatosis and hepatotoxicity-associated events.01A - Beitrag in wissenschaftlicher ZeitschriftPublikation Plasmonic photothermal activation of an organosilica shielded cold-adapted lipase co-immobilised with gold nanoparticles on silica particles(Royal Society of Chemistry, 01.01.2023) Giunta, Carolina; Nazemi, Seyed Amirabbas; Olesińska, Magdalena; Shahgaldian, PatrickGold nanoparticles (AuNPs), owing to their intrinsic plasmonic properties, are widely used in applications ranging from nanotechnology and nanomedicine to catalysis and bioimaging. Capitalising on the ability of AuNPs to generate nanoscale heat upon optical excitation, we designed a nanobiocatalyst with enhanced cryophilic properties. It consists of gold nanoparticles and enzyme molecules, co-immobilised onto a silica scaffold, and shielded within a nanometre-thin organosilica layer. To produce such a hybrid system, we developed and optimized a synthetic method allowing efficient AuNP covalent immobilisation on the surface of silica particles (SPs). Our procedure allows to reach a dense and homogeneous AuNP surface coverage. After enzyme co-immobilisation, a nanometre-thin organosilica layer was grown on the surface of the SPs. This layer was designed to fulfil the dual function of protecting the enzyme from the surrounding environment and allowing the confinement, at the nanometre scale, of the heat diffusing from the AuNPs after surface plasmon resonance photothermal activation. To establish this proof of concept, we used an industrially relevant lipase enzyme, namely Lipase B from Candida Antarctica (CalB). Herein, we demonstrate the possibility to photothermally activate the so-engineered enzymes at temperatures as low as −10 °C.01A - Beitrag in wissenschaftlicher ZeitschriftPublikation Method to rapidly identify potential solvent systems for crystallization of cocrystals(American Chemical Society, 2023) Codan, Lorenzo; Daza Olivella, Laura; Sirota, Eric01A - Beitrag in wissenschaftlicher ZeitschriftPublikation Carbon capture from flue gas of a chemical industrial park(Hochschule für Life Sciences FHNW, 2023) Furrer, Thierry; Zogg, Andreas; GetecIn the chemical park Schweizerhalle, multiple incineration plants handle the hazardous waste generated by production processes. The ALV1 is the smaller one of the two waste solvent incinerators. Within the scope of this project, the feasibility to upgrade this plant with a carbon capture unit was assessed.11 - Studentische Arbeit