Lipps, Georg

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Georg
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Lipps, Georg

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2015 - 20195
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Enddatum: 2019 × Dateien dazu vorhanden?: Nein ×

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    Publikation
    Characterization of the housekeeping sortase from the human pathogen Propionibacterium acnes - first investigation of a class F sortase
    (Portland Press, 2019) Di Girolamo, Salvatore; Lipps, Georg [in: Biochemical Journal]
    Sortase enzymes play an important role in Gram-positive bacteria. They are responsible for the covalent attachment of proteins to the surface of the bacteria and perform this task via a highly sequence-specific transpeptidation reaction. Since these immobilized proteins are often involved in pathogenicity of Gram-positive bacteria, characterization of this type of enzyme is also of medical relevance. Different classes of sortases (A-F) have been found, which recognize characteristic recognition sequences present in substrate proteins. Up to date, sortase A from Staphylococcus aureus, a housekeeping class A sortase, is the most thoroughly studied representative of the sortase family of enzymes. Here we report the in-depth characterization of the class F sortase from Propionibacterium acnes, a class of sortases that has not been investigated before. As Sortase F is the only transpeptidase found in the P. acnes genome, it is the housekeeping sortase of this organism. Sortase F from P. acnes shows a behavior similar to sortases from class A in terms of pH dependence, recognition sequence and catalytic activity; furthermore, its activity is independent of bivalent ions, which contrasts to sortase A from S. aureus We demonstrate that sortase F is useful for protein engineering applications, by producing a site-specifically conjugated homogenous antibody-drug conjugate with a potency similar to that of a conjugate prepared with sortase A. Thus, the detailed characterization presented here will not only enable the development of anti-virulence agents targeting P. acnes but also provides a powerful alternative to sortase A for protein engineering applications. © 2019 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society.
    01A - Beitrag in wissenschaftlicher Zeitschrift
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    Publikation
    A Small Helical Bundle Prepares Primer Synthesis by Binding Two Nucleotides that Enhance Sequence-Specific Recognition of the DNA Template
    (Cell Press, 27.12.2018) Boudet, Julien; Devillier, Jean-Christophe; Lipps, Georg [in: Cell]
    01A - Beitrag in wissenschaftlicher Zeitschrift
  • Vorschaubild nicht verfügbar
    Publikation
    Evidence-Based Clinical Use of Nanoscale Extracellular Vesicles in Nanomedicine
    (American Chemical Society, 26.04.2016) Lipps, Georg [in: ACS Nano]
    Recent research has demonstrated that all body fluids assessed contain substantial amts. of vesicles that range in size from 30 to 1000 nm and that are surrounded by phospholipid membranes contg. different membrane microdomains such as lipid rafts and caveolae. The most prominent representatives of these so-​called extracellular vesicles (EVs) are nanosized exosomes (70-​150 nm)​, which are derivs. of the endosomal system, and microvesicles (100-​1000 nm)​, which are produced by outward budding of the plasma membrane. Nanosized EVs are released by almost all cell types and mediate targeted intercellular communication under physiol. and pathophysiol. conditions. Contg. cell-​type-​specific signatures, EVs have been proposed as biomarkers in a variety of diseases. Furthermore, according to their phys. functions, EVs of selected cell types have been used as therapeutic agents in immune therapy, vaccination trials, regenerative medicine, and drug delivery. Undoubtedly, the rapidly emerging field of basic and applied EV research will significantly influence the biomedicinal landscape in the future. In this Perspective, we, a network of European scientists from clin., academic, and industry settings collaborating through the H2020 European Cooperation in Science and Technol. (COST) program European Network on Microvesicles and Exosomes in Health and Disease (ME-​HAD)​, demonstrate the high potential of nanosized EVs for both diagnostic and therapeutic (i.e., theranostic) areas of nanomedicine.
    01A - Beitrag in wissenschaftlicher Zeitschrift
  • Vorschaubild nicht verfügbar
    Publikation
    Biological properties of extracellular vesicles and their physiological functions
    (Taylor & Francis, 14.05.2015) Lipps, Georg [in: Journal of Extracellular Vesicles]
    01A - Beitrag in wissenschaftlicher Zeitschrift
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    Publikation
    Structures to complement the archaeo-eukaryotic primases catalytic cycle description: What's next?
    (Elsevier, 02.05.2015) Boudet, Julien; Devillier, Jean-Christophe; Allain, Frédéric H.-T.; Lipps, Georg [in: Computational and Structural Biotechnology Journal]
    01A - Beitrag in wissenschaftlicher Zeitschrift