Cytotoxic effects of pentachlorophenol (PCP) and its metabolite tetrachlorohydroquinone (TCHQ) on rainbow trout liver cells are modulated by antioxidants
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Dateien
Autor:innen
Autor:in (Körperschaft)
Publikationsdatum
05.07.2014
Typ der Arbeit
Studiengang
Typ
01A - Beitrag in wissenschaftlicher Zeitschrift
Herausgeber:innen
Herausgeber:in (Körperschaft)
Betreuer:in
Übergeordnetes Werk
Cell Biology and Toxicology
Themenheft
DOI der Originalpublikation
Link
Reihe / Serie
Reihennummer
Jahrgang / Band
Ausgabe / Nummer
30
Seiten / Dauer
233-252
Patentnummer
Verlag / Herausgebende Institution
Springer
Verlagsort / Veranstaltungsort
Dodrecht
Auflage
Version
Programmiersprache
Abtretungsempfänger:in
Praxispartner:in/Auftraggeber:in
Zusammenfassung
Abstract
The world-wide distribution and high bioaccumulation potential of pentachlorophenol (PCP) in aquatic organisms imply a high toxicological impact in aquatic systems. Firstly, our investigations show that, similar to mammalian cell lines, PCP can be metabolized to tetrachlorohydroquinone (TCHQ) in the permanent cell line derived from rainbow trout liver cells (RTL W-1). Moreover, we demonstrate that PCP as well as its metabolite TCHQ is capable of influencing the viability of these cells. Three cell viability assays were performed to assess possible cellular targets of these substances. Thus, the toxicity of the PCP-derivative TCHQ was shown for the first time in a fish cell line. Further investigations revealed the involvement of ROS in the cytotoxicity of PCP and its metabolite TCHQ. The observation of oxidative stress due to PCP or TCHQ exposure does provide a plausible explanation for the increased cytotoxicity of higher concentrations of both substances and implies possible mechanisms underlying these observations. In addition, antioxidants such as ascorbic acid and quercetin modulate the detrimental effects of PCP and TCHQ whereby both compounds exacerbate the cytotoxic effects of high PCP and TCHQ concentrations.
Schlagwörter
antioxidants, ascorbate, cytotoxicity, oxidative stress, pentachlorophenol – PCP, quercetin, tetrachlorohydroquinone – TCHQ
Fachgebiet (DDC)
570 - Biowissenschaften, Biologie
540 - Chemie
540 - Chemie
Veranstaltung
Startdatum der Ausstellung
Enddatum der Ausstellung
Startdatum der Konferenz
Enddatum der Konferenz
Datum der letzten Prüfung
ISBN
ISSN
0742-2091
1573-6822
1573-6822
Sprache
Englisch
Während FHNW Zugehörigkeit erstellt
Ja
Publikationsstatus
Veröffentlicht
Begutachtung
Peer-Review der ganzen Publikation
Open Access-Status
Lizenz
Zitation
PIETSCH, Constanze, Juliane HOLLENDER, Patricia BURKHARDT-HOLM und Falk DORUSCH, 2014. Cytotoxic effects of pentachlorophenol (PCP) and its metabolite tetrachlorohydroquinone (TCHQ) on rainbow trout liver cells are modulated by antioxidants. Cell Biology and Toxicology. 5 Juli 2014. Nr. 30, S. 233–252. DOI 10.26041/fhnw-169. Verfügbar unter: https://doi.org/10.26041/fhnw-169