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dc.contributor.authorWedler, J
dc.contributor.authorRusanov, K
dc.contributor.authorAtanassov, I
dc.contributor.authorButterweck, Veronika
dc.date.accessioned2016-11-09T11:07:40Z
dc.date.available2016-11-09T11:07:40Z
dc.date.issued2016
dc.identifier.issn0032-0943
dc.identifier.doi10.1055/s-0042-105158
dc.identifier.urihttp://hdl.handle.net/11654/23427
dc.description.abstractWater steam distillation of rose flowers separates the essential oil from the polyphenol-containing rose oil distillation wastewater. Recently, a strategy was developed to separate rose oil distillation wastewater into a polyphenol depleted water fraction and a polyphenol-enriched fraction [RF20-(SP-207)]. The objective of the present study was to investigate RF20-(SP-207) and fraction F(IV), augmented in quercetin and ellagic acid, for possible antiproliferative effects in immortalized human keratinocytes (HaCaT) since rose petals are known to contain compounds with potential antiproliferative activity.RF20-(SP-207) revealed dose-dependent antiproliferative activity (IC50 of 9.78 µg/mL). In a nontoxic concentration of 10 µg/mL, this effect was stronger than that of the two positive controls LY294002 (10 µM, PI3 K-inhibitor, 30 % inhibition) and NVP-BEZ235 (100 nM, dual PI3 K/mTOR inhibitor, 30 % inhibition) and clearly exceeded the antiproliferative action of quercetin (50 µM, 25 % inhibition) and ellagic acid (1 µM, 15 % inhibition). Time-lapse microscopy detected a significant impairment of cell migration of RF20-(SP-207) and F(IV). At concentrations of 10 µg/mL of both, extract and fraction, cell migration was strongly suppressed (51 % and 28 % gap closure, respectively, compared to 95 % gap closure 24 hours after control treatment). The suppression of cell migration was comparable to the positive controls LY294002, NVP-BEZ235, and quercetin. Furthermore, basal and TNF-α-stimulated VEGF-secretion was significantly reduced by RF20-(SP-207) and F(IV) at 10 µg/mL (44 % vs. untreated control).In conclusion, RF20-(SP-207) showed promising antiproliferative and antimigratory effects and could be developed as a supportive, therapy against hyperproliferation-involved skin diseases.
dc.description.urihttps://www.ncbi.nlm.nih.gov/pubmed/27093251
dc.language.isoen
dc.relation.ispartofPlanta Medica
dc.accessRightsAnonymous
dc.subjectRosa damascene
dc.subjectwastewater
dc.subjectquercetin
dc.subjectRosaceae
dc.subjectellagic acid
dc.subjectcell proliferation
dc.subjectVEGF
dc.titleA polyphenol-enriched fraction of rose oil distillation wastewater inhibits cell proliferation, migration and TNF-α-Induced VEGF secretion in human immortalized keratinocytes
dc.type01 - Zeitschriftenartikel, Journalartikel oder Magazin
dc.volume82
dc.issue11-12
dc.audienceScience
fhnw.publicationStatePublished
fhnw.ReviewTypeAnonymous ex ante peer review of a complete publication
fhnw.InventedHereYes
fhnw.PublishedSwitzerlandNo
fhnw.pagination1000-1008
fhnw.IsStudentsWorkno
fhnw.publicationOnlineJa


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