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    Biotransformation of ritalinic acid by laccase in the presence of mediator TEMPO

    Datum
    07.2018
    Autorin/Autor
    Kobakhidze, Aza
    Elisashvili, Vladimir
    Corvini, Philippe
    Cvancarova Småstuen, M.
    Metadata
    Zur Langanzeige
    Type
    01 - Zeitschriftenartikel, Journalartikel oder Magazin
    Primary target group
    Science
    Created while belonging to FHNW?
    Yes
    Zusammenfassung
    Methylphenidate is widely used as a medication for the treatment of attention deficit hyperactivity disorder (ADHD) in children. Less than 1% of methylphenidate is excreted unchanged in urine, while 80% of an oral dose is excreted as ritalinic acid (which is reportedly poorly degradable). This study aims to investigate the biotransformation of ritalinic acid by free and immobilized enzymes. The influence of various laccase mediators on biotransformation efficiency has been tested. Formation of the main transformation products has been monitored and their potential structures suggested. The effective transformation of ritalinic acid was observed only in the presence of 2,2,6,6-tetramethylpiperidine 1-oxyl mediator (TEMPO). The most effective enzyme was the laccase of T. versicolor 159. The main transformation product was an N-methyl derivative of ritalinic acid. Ritalinic acid was also reduced to aldehyde and alcohol, and a broad spectrum of intermediate complexes with oxoammonium ion of TEMPO were detected. This is the first time the biotransformation of ritalinic acid has been investigated in detail.
    URI
    http://hdl.handle.net/11654/27321

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