Koehler, F. M.Raso, RenzoGrass, R. N.Stark, W. J.2024-09-122024-09-122013-05-131613-68101613-682910.1002/smll.201300925https://irf.fhnw.ch/handle/11654/47283Immunoassays are used for detecting protein targets for various applications. Here, a modification of immunoassays to allow a purely electrical detection of the target protein concentration is shown. The modification comprises a β-D-glucosidase as reporter enzyme and a cyanogenic glycoside as substrate. The enzymatic reaction produces cyanide in small quantities. For electrical detection of the cyanide, a novel sensor is developed, based on a gold micro wire. The cyanide dissolves the gold wire and changes the electrical resistance of the wire. Monitoring the resistance change allows a quantitative measurement of the target human C-reactive protein (an inflammatory marker) in blood plasma in the physiological relevant concentration range.en500 - Naturwissenschaften und Mathematikβ‐D‐glucosidase assisted gold dissolution as non‐optical and quantifiable detection technique for immunoassays01A - Beitrag in wissenschaftlicher Zeitschrift4000-4005