Institut für Chemie und Bioanalytik
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Auflistung Institut für Chemie und Bioanalytik nach Schlagwort "600 - Technik, Medizin, angewandte Wissenschaften"
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- Publikation3D printed microfluidic modules. Passive mixers and cells encapsulation in alginate(De Gruyter, 02.09.2022) Dalcanale, Federico; Caj, Michaela; Schuler, Felix; Ganeshanathan, Kireedan; Suter-Dick, Laura [in: Current Directions in Biomedical Engineering]Passive mixers and droplet generation microfluidic chip modules were designed and manufactured using a commercial SLA 3D-printer. The mixing modules were designed specifically for 3D-printing and evaluated using FEM modeling. The co-flow droplet generator was used for cancer cells encapsulation and drug potency evaluation.01A - Beitrag in wissenschaftlicher Zeitschrift
- PublikationAbsorption and metabolism of the natural sweeteners erythritol and xylitol in humans. A dose-ranging study(MDPI, 30.08.2022) Wölnerhanssen, Bettina K.; Meyer-Gerspach, Anne Christin; Bordier, Valentine; Teysseire, Valentine; Senner, Frank; Schlotterbeck, Götz; Drewe, Jürgen; Beglinger, Christoph [in: International Journal of Molecular Sciences]The natural sweeteners erythritol and xylitol might be helpful to reduce sugar consumption and therefore prevent obesity and diabetes. The aim of the present study was to determine the absorption and metabolization into erythronate of different concentrations of erythritol and xylitol. Seventeen healthy lean participants received intragastric solutions of 10, 25, or 50 g erythritol or 7, 17, or 35 g xylitol on three study days in a randomized order. The study was double blinded with respect to the doses administered. We assessed plasma concentrations of erythritol, xylitol, and erythronate at fixed time intervals after administration with gas chromatography-mass spectrometry. We found: (i) a dose-dependent and saturable absorption of erythritol, (ii) a very low absorption of xylitol, (iii) a dose-dependent metabolization of erythritol into erythronate, and (iv) no metabolization of xylitol into erythronate. The implications of the metabolization of erythritol into erythronate for human health remain to be determined and more research in this area is needed.01A - Beitrag in wissenschaftlicher Zeitschrift
- PublikationAllosteric targeting resolves limitations of earlier LFA-1 directed modalities(Elsevier, 05/2023) Mancuso, Riccardo V.; Schneider, Gisbert; Hürzeler Müller, Marianne; Gut, Martin; Zurflüh, Jonas; Breitenstein, Werner; Bouitbir, Jamal; Reisen, Felix; Atz, Kenneth; Ehrhardt, Claus; Duthaler, Urs; Gygax, Daniel; Schmidt, Albrecht G.; Krähenbühl, Stephan; Weitz-Schmidt, Gabriele [in: Biochemical Pharmacology]01A - Beitrag in wissenschaftlicher Zeitschrift
- PublikationAn origami like 3D patterned cellulose-based scaffold for bioengineering cardiovascular applications(Springer, 2023) Rodriguez, Gabriela Melo; Trueb, Donata; Köser, Joachim; Schoelkopf, Joachim; Gullo, Maurizio [in: Cellulose]In this work we describe the manufacturing of cellulosic, cell compatible scaffolds with an inherent 3D origami crease pattern for applications in cardiac tissue engineering. Different cellulosic materials were studied, among them cotton linters, fibers obtained from eucalyptus, pine, spruce and lyocell. Formed sheets made of cotton linters were chosen for further study due to the highest biocompatibility and mechanical properties best suited for cardiomyocytes in wet and dry conditions: E - modulus of 0.8 GPa, tensile strength of 4.7 MPa and tensile strength in wet environment of 2.28 MPa. Cell alignment is desired to achieve directional contraction of the cardiac tissue, and several options were investigated to achieve fiber alignment, e.g. a dynamic sheet former and Rapid Köthen sheet former. Although the orientation was minimal, cells cultured on the cellulose fibers grew and aligned along the fibers. Origami inspired crease patterns were applied to the cellulose scaffolds in order to introduce directional flexibility beneficial for cardiac contraction. The transfer of a Miura-ori crease pattern was successfully applied in two ways: folding of the dried sheet between PET foils pre-formed in a 3D printed mold, and in situ wet fiber molding on a 3D-patterned mesh mounted in the sheet former’s sieve section. The latter approach enables upscaling for potential mass production.01A - Beitrag in wissenschaftlicher Zeitschrift
- PublikationApical medium flowiInfluences the morphology and physiology of human proximal tubular cells in a microphysiological system(MDPI, 30.09.2022) Specioso, Gabriele; Bovard, David; Zanetti, Filippo; Maranzano, Fabio; Merg, Céline; Sandoz, Antonin; Titz, Bjoern; Dalcanale, Federico; Hoeng, Julia; Renggli, Kasper; Suter-Dick, Laura [in: Bioengineering]There is a lack of physiologically relevant in vitro human kidney models for disease modelling and detecting drug-induced effects given the limited choice of cells and difficulty implementing quasi-physiological culture conditions. We investigated the influence of fluid shear stress on primary human renal proximal tubule epithelial cells (RPTECs) cultured in the micro-physiological Vitrofluid device. This system houses cells seeded on semipermeable membranes and can be connected to a regulable pump that enables controlled, unidirectional flow. After 7 days in culture, RPTECs maintained physiological characteristics such as barrier integrity, protein uptake ability, and expression of specific transporters (e.g., aquaporin-1). Exposure to constant apical side flow did not cause cytotoxicity, cell detachment, or intracellular reactive oxygen species accumulation. However, unidirectional flow profoundly affected cell morphology and led to primary cilia lengthening and alignment in the flow direction. The dynamic conditions also reduced cell proliferation, altered plasma membrane leakiness, increased cytokine secretion, and repressed histone deacetylase 6 and kidney injury molecule 1 expression. Cells under flow also remained susceptible to colistin-induced toxicity. Collectively, the results suggest that dynamic culture conditions in the Vitrofluid system promote a more differentiated phenotype in primary human RPTECs and represent an improved in vitro kidney model.01A - Beitrag in wissenschaftlicher Zeitschrift
- PublikationCanIsoNet: a database to study the functional impact of isoform switching events in diseases(Oxford University Press, 17.04.2023) Karakulak, Tülay; Szklarczyk, Damian; Saylan, Cemil Can; Moch, Holger; von Mering, Christian; Kahraman, Abdullah; Ouangraoua, Aida [in: Bioinformatics Advances]Motivation: Alternative splicing, as an essential regulatory mechanism in normal mammalian cells, is frequently disturbed in cancer and other diseases. Switches in the expression of most dominant alternative isoforms can alter protein interaction networks of associated genes giving rise to disease and disease progression. Here, we present CanIsoNet, a database to view, browse and search isoform switching events in diseases. CanIsoNet is the first webserver that incorporates isoform expression data with STRING interaction networks and ClinVar annotations to predict the pathogenic impact of isoform switching events in various diseases. Results: Data in CanIsoNet can be browsed by disease or searched by genes or isoforms in annotation-rich data tables. Various annotations for 11 811 isoforms and 14 357 unique isoform switching events across 31 different disease types are available. The network density score for each disease-specific isoform, PFAM domain IDs of disrupted interactions, domain structure visualization of transcripts and expression data of switched isoforms for each sample is given. Additionally, the genes annotated in ClinVar are highlighted in interactive interaction networks. Availability and implementation: CanIsoNet is freely available at https://www.caniso.net. The source codes can be found under a Creative Common License at https://github.com/kahramanlab/CanIsoNet_Web.01A - Beitrag in wissenschaftlicher Zeitschrift
- PublikationComputationally driven discovery of SARS-CoV-2 Mpro inhibitors. From design to experimental validation(Royal Society of Chemistry, 01.01.2022) El Khoury, Léa; Jing, Zhifeng; Cuzzolin, Alberto; Deplano, Alessandro; Loco, Daniele; Sattarov, Boris; Hédin, Florent; Ho, Chris; El Ahdab, Dina; Jaffrelot Inizan, Theo; Sturlese, Mattia; Sosic, Alice; Volpiana, Martina; Lugato, Angela; Barone, Marco; Gatto, Barbara; Macchia, Maria Ludovica; Bellanda, Massimo; Battistutta, Roberto; Salata, Cristiano; Kondratov, Ivan; Iminov, Rustam; Khairulin, Andrii; Mykhalonok, Yaroslav; Pochepko, Anton; Chashka-Ratushnyi, Volodymyr; Kos, Iaroslava; Moro, Stefano; Montes, Matthieu; Ren, Pengyu; Ponder, Jay W.; Lagardère, Louis; Piquemal, Jean-Philip; Sabbadin, Davide; Wendeborn, Sebastian [in: Chemical Science]We report a fast-track computationally driven discovery of new SARS-CoV-2 main protease (Mpro) inhibitors whose potency ranges from mM for the initial non-covalent ligands to sub-μM for the final covalent compound (IC50 = 830 ± 50 nM). The project extensively relied on high-resolution all-atom molecular dynamics simulations and absolute binding free energy calculations performed using the polarizable AMOEBA force field. The study is complemented by extensive adaptive sampling simulations that are used to rationalize the different ligand binding poses through the explicit reconstruction of the ligand–protein conformation space. Machine learning predictions are also performed to predict selected compound properties. While simulations extensively use high performance computing to strongly reduce the time-to-solution, they were systematically coupled to nuclear magnetic resonance experiments to drive synthesis and for in vitro characterization of compounds. Such a study highlights the power of in silico strategies that rely on structure-based approaches for drug design and allows the protein conformational multiplicity problem to be addressed. The proposed fluorinated tetrahydroquinolines open routes for further optimization of Mpro inhibitors towards low nM affinities.01A - Beitrag in wissenschaftlicher Zeitschrift
- PublikationDetermination of active ingredients in formulated plant protection products by UHPLC-UV/MS(Springer, 04.10.2022) Erdin, Yves; Schlotterbeck, Götz; Mink, Christian [in: Journal of Consumer Protection and Food Safety]The market for plant protection products (PPPs) is one of the most regulated. With increasing regulations for registering PPPs, its requirements take more effort and more testing. Meanwhile, there is also an increasing market for illegal PPPs. While legally registered PPPs are thoroughly tested and documented, illegal PPPs are sold upfront without any testing (e.g., tox, aqua tox, eco tox). This bears additional risk for the users, the ecosystem and the public. There are many well established analytical methods and protocols to analyse soil, plant products as well as for food and beverage, but less for PPPs. Many methods need laborious sample preparation and extraction steps like QuEChERS and/or advanced mass spectrometry techniques. Here we present a method developed to identify active ingredients (AIs) which is easy to apply at relatively cheap costs and widely available instrumentation. It provides a straightforward set-up for sample preparation and analysis, without the need for elaborative sample preparation. The method validation showed sufficient linearity, repeatability and specificity to use this method for screening of samples taken from market control for counterfeit or cross contamination checks.01A - Beitrag in wissenschaftlicher Zeitschrift
- PublikationEnhanced formation of nanometric titanium cones by incorporation of titanium, tungsten and/or iron in a helium ion beam(Elsevier, 12/2022) Sanchez, Fabien; Steiner, Roland; Latttner, P.; Spicher, J.; Mathys, Daniel; Antunes, Rodrigo; Marcin, Kisiel; Mukkadam, Khaled; Astasov-Fraunhofer, Monika; Kühl, Sebastian; Köser, Joachim; Marot, Laurent; Meyer, Ernst [in: Surfaces and Interfaces]Surface patterning of bio-compatible titanium (Ti) shows a growing interest in the medical field. The engineering of material surfaces can achieve bactericidal properties and osteointegration improvements in order to develop medical implants. Spikes-like surface morphologies have already demonstrated the development of promising bactericidal properties. A barely new method to produce nanometric-sized cones on titanium consists of helium (He) ion irradiation using low energies ( 100 eV) and temperatures comprised between 0.25 T/T 0.5 (with T being the melting temperature of the material). Ti, iron (Fe) and/or tungsten (W) were incorporated in a He beam, and their amounts were quantified using X-ray Photoelectron Spectroscopy (XPS). The He ion energy was varied from 70 and 120 eV, the surface temperatures from 571 to 651 K for fluences approximately equal to 1024 m−2. After irradiation, the surface morphology was characterized using Scanning Electron Microscopy (SEM) and Focused Ion Beam (FIB). This study demonstrated the capability for irradiated Ti surfaces to form cones with tunable density, aspect ratio, and heights with the incorporation of Ti, Fe and/or W in a He ion. Additionally, the growth rate of the cones was enhanced by about 30 times in comparison to pure He irradiation as a function of the chosen materials introduced in the He beam.01A - Beitrag in wissenschaftlicher Zeitschrift
- PublikationIsoAligner. Dynamic mapping of amino acid positions across protein isoforms(Faculty of 1000, 31.03.2022) Hanimann, Jacob; Moch, Holger; Zoche, Martin; Kahraman, Abdullah [in: F1000Research]Aligning protein isoform sequences is often performed in cancer diagnostics to homogenise mutation annotations from different diagnostic assays. However, most alignment tools are fitted for homologous sequences, leading often to alignments of non-identical exonic regions. Here, we present the interactive alignment webservice IsoAligner for exact mapping of exonic protein subsequences. The tool uses a customized Needleman-Wunsch algorithm including an open gap penalty combined with a gene-specific minimal exon length function and dynamically adjustable parameters. As an input, IsoAligner accepts either various gene/transcript/protein IDs from different databases (Ensembl, UniProt, RefSeq) or raw amino acid sequences. The output of IsoAligner consists of pairwise alignments and a table of mapped amino acid positions between the canonical or supplied isoform IDs and all alternative isoforms. IsoAligner’s human isoform library comprises of over 1.3 million IDs mapped on over 120,000 protein sequences. IsoAligner, is a fast and interactive alignment tool for retrieving amino acids positions between different protein isoforms. Its application will allow diagnostic and precision medicine labs to detect inconsistent variant annotations between different assays and databases. Availability: This tool is available as a Webservice on www.isoaligner.org. A REST API is available for programmatic access. The source code for both services can be found at https://github.com/mtp-usz/IsoAligner.01A - Beitrag in wissenschaftlicher Zeitschrift
- PublikationMTPpilot. An interactive software for visualization of next-generation sequencing results in molecular tumor boards(American Society of Clinical Oncology Journal, 25.08.2022) Arnold, Fabian M.; Hanimann, Jacob; Nowak, Marta; Pauli, Chantal; Britschgi, Christian; Moch, Holger; Zoche, Martin; Kahraman, Abdullah [in: JCO Clinical Cancer Informatics]01A - Beitrag in wissenschaftlicher Zeitschrift
- PublikationPlasmonic photothermal activation of an organosilica shielded cold-adapted lipase co-immobilised with gold nanoparticles on silica particles(Royal Society of Chemistry, 01.01.2023) Giunta, Carolina; Nazemi, Seyed Amirabbas; Olesińska, Magdalena; Shahgaldian, Patrick [in: Nanoscale Advances]Gold nanoparticles (AuNPs), owing to their intrinsic plasmonic properties, are widely used in applications ranging from nanotechnology and nanomedicine to catalysis and bioimaging. Capitalising on the ability of AuNPs to generate nanoscale heat upon optical excitation, we designed a nanobiocatalyst with enhanced cryophilic properties. It consists of gold nanoparticles and enzyme molecules, co-immobilised onto a silica scaffold, and shielded within a nanometre-thin organosilica layer. To produce such a hybrid system, we developed and optimized a synthetic method allowing efficient AuNP covalent immobilisation on the surface of silica particles (SPs). Our procedure allows to reach a dense and homogeneous AuNP surface coverage. After enzyme co-immobilisation, a nanometre-thin organosilica layer was grown on the surface of the SPs. This layer was designed to fulfil the dual function of protecting the enzyme from the surrounding environment and allowing the confinement, at the nanometre scale, of the heat diffusing from the AuNPs after surface plasmon resonance photothermal activation. To establish this proof of concept, we used an industrially relevant lipase enzyme, namely Lipase B from Candida Antarctica (CalB). Herein, we demonstrate the possibility to photothermally activate the so-engineered enzymes at temperatures as low as −10 °C.01A - Beitrag in wissenschaftlicher Zeitschrift
- PublikationROS1 genomic rearrangements are rare actionable drivers in microsatellite stable colorectal cancer(Wiley, 30.08.2022) Akhoundova, Dilara; Hussung, Saskia; Sivakumar, Smruthy; Töpfer, Antonia; Rechsteiner, Markus; Arnold, Fabian; Angst, Florian; Britschgi, Christian; Zoche, Martin; Moch, Holger; Weber, Achim; Sokol, Ethan; Fritsch, Ralph M.; Kahraman, Abdullah [in: International Journal of Cancer]01A - Beitrag in wissenschaftlicher Zeitschrift
- PublikationSARS-CoV-2 variants of concern and clinical severity in the mexican pediatric population(MDPI, 2023) Maldonado-Cabrera, Anahí; Colin-Vilchis, Jesus Alejandro; Haque, Ubydul; Velazquez, Carlos; Alvarez Villaseñor, Andrea Socorro; Magdaleno-Márquez, Luis Eduardo; Calleros-Muñoz, Carlos Iván; Figueroa-Enríquez, Karen Fernanda; Angulo-Molina, Aracely; Gallego-Hernández, Ana Lucía [in: Infectious Disease Reports]The emergence of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) variants of concern (VOCs) presents global heterogeneity, and their relative effect on pediatric severity is still limited. In this study, we associate VOCs with pediatric clinical severity outcomes in Mexico. Bioinformatics methods were used to characterize VOCs and single amino acid (aa) mutations in 75,348 SARS-CoV-2 genetic sequences from February 2020 to October 2022. High-predominance VOCs groups were calculated and subsequently associated with 372,989 COVID-19 clinical pediatric outcomes. We identified 21 high-frequency mutations related to Omicron lineages with an increased prevalence in pediatric sequences compared to adults. Alpha and the other lineages had a significant increase in case fatality rate (CFR), intensive critical unit (ICU) admission, and automated mechanical ventilation (AMV). Furthermore, a logistic model with age-adjusted variables estimated an increased risk of hospitalization, ICU/AMV, and death in Gamma and Alpha, in contrast to the other lineages. We found that, regardless of the VOCs lineage, infant patients presented the worst severity prognoses. Our findings improve the understanding of the impact of VOCs on pediatric patients across time, regions, and clinical outcomes. Enhanced understanding of the pediatric severity for VOCs would enable the development and improvement of public health strategies worldwide.01A - Beitrag in wissenschaftlicher Zeitschrift
- PublikationStochastic chain termination in bacterial pilus assembly(Nature, 2023) Giese, Christoph; Puorger, Chasper; Ignatov, Oleksandr; Bečárová, Zuzana; Weber, Marco E.; Schärer, Martin A.; Capitani, Guido; Glockshuber, Rudi [in: Nature Communications]Adhesive type 1 pili from uropathogenic Escherichia coli strains are filamentous, supramolecular protein complexes consisting of a short tip fibrillum and a long, helical rod formed by up to several thousand copies of the major pilus subunit FimA. Here, we reconstituted the entire type 1 pilus rod assembly reaction in vitro, using all constituent protein subunits in the presence of the assembly platform FimD, and identified the so-far uncharacterized subunit FimI as an irreversible assembly terminator. We provide a complete, quantitative model of pilus rod assembly kinetics based on the measured rate constants of FimD-catalyzed subunit incorporation. The model reliably predicts the length distribution of assembled pilus rods as a function of the ratio between FimI and the main pilus subunit FimA and is fully consistent with the length distribution of membrane-anchored pili assembled in vivo. The results show that the natural length distribution of adhesive pili formed via the chaperone-usher pathway results from a stochastic chain termination reaction. In addition, we demonstrate that FimI contributes to anchoring the pilus to the outer membrane and report the crystal structures of (i) FimI in complex with the assembly chaperone FimC, (ii) the FimI-FimC complex bound to the N-terminal domain of FimD, and (iii) a ternary complex between FimI, FimA and FimC that provides structural insights on pilus assembly termination and pilus anchoring by FimI.01A - Beitrag in wissenschaftlicher Zeitschrift
- PublikationSynthetic phosphoethanolamine-modified oligosaccharides reveal the importance of glycan length and substitution in biofilm-inspired assemblies(Nature, 08.07.2022) Tyrikos-Ergas, Theodore; Gim, Soeun; Huang, Jhih-Yi; Pinzón Martín, Sandra; Varon, Daniel; Seeberger, Peter H.; Delbianco, Martina [in: Nature Communications]Bacterial biofilm matrices are nanocomposites of proteins and polysaccharides with remarkable mechanical properties. Efforts understanding and tuning the protein component have been extensive, whereas the polysaccharide part remained mostly overlooked. The discovery of phosphoethanolamine (pEtN) modified cellulose in biofilms revealed that polysaccharide functionalization alters the biofilm properties. To date, the pattern of pEtN cellulose and its mode of interactions with proteins remains elusive. Herein, we report a model system based on synthetic epitomes to explore the role of pEtN in biofilm-inspired assemblies. Nine pEtN-modified oligosaccharides were synthesized with full control over the length, degree and pattern of pEtN substitution. The oligomers were co-assembled with a representative peptide, triggering the formation of fibers in a length dependent manner. We discovered that the pEtN pattern modulates the adhesion of biofilm-inspired matrices, while the peptide component controls its stiffness. Unnatural oligosaccharides tune or disrupt the assembly morphology, revealing interesting targets for polysaccharide engineering to develop tunable bio-inspired materials.01A - Beitrag in wissenschaftlicher Zeitschrift
- PublikationTailoring a solvent-assisted method for solid-supported hybrid lipid–polymer membranes(American Chemical Society, 17.05.2022) Di Leone, Stefano; Kyropoulou, Myrto; Köchlin, Julian; Wehr, Riccardo; Meier, Wolfgang P.; Palivan, Cornelia G. [in: Langmuir]Combining amphiphilic block copolymers and phospholipids opens new opportunities for the preparation of artificial membranes. The chemical versatility and mechanical robustness of polymers together with the fluidity and biocompatibility of lipids afford hybrid membranes with unique properties that are of great interest in the field of bioengineering. Owing to its straightforwardness, the solvent-assisted method (SA) is particularly attractive for obtaining solid-supported membranes. While the SA method was first developed for lipids and very recently extended to amphiphilic block copolymers, its potential to develop hybrid membranes has not yet been explored. Here, we tailor the SA method to prepare solid-supported polymer–lipid hybrid membranes by combining a small library of amphiphilic diblock copolymers poly(dimethyl siloxane)–poly(2-methyl-2-oxazoline) and poly(butylene oxide)-block–poly(glycidol) with phospholipids commonly found in cell membranes including 1,2-dihexadecanoyl-sn-glycero-3-phosphocholine, 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoethanolamine, sphingomyelin, and 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(glutaryl). The optimization of the conditions under which the SA method was applied allowed for the formation of hybrid polymer–lipid solid-supported membranes. The real-time formation and morphology of these hybrid membranes were evaluated using a combination of quartz crystal microbalance and atomic force microscopy. Depending on the type of polymer–lipid combination, significant differences in membrane coverage, formation of domains, and quality of membranes were obtained. The use of the SA method for a rapid and controlled formation of solid-supported hybrid membranes provides the basis for developing customized artificial hybrid membranes.01A - Beitrag in wissenschaftlicher Zeitschrift
- PublikationUsing GPCRs as molecular beacons to target ovarian cancer with nanomedicines(MDPI, 10.05.2022) Khetan, Riya; Dharmayanti, Cintya; Gillam, Todd A.; Kübler, Eric; Klingler-Hoffmann, Manuela; Ricciardelli, Carmela; Oehler, Martin K.; Blencowe, Anton; Garg, Sanjay; Albrecht, Hugo [in: Cancers]The five-year survival rate for women with ovarian cancer is very poor despite radical cytoreductive surgery and chemotherapy. Although most patients initially respond to platinum-based chemotherapy, the majority experience recurrence and ultimately develop chemoresistance, resulting in fatal outcomes. The current administration of cytotoxic compounds is hampered by dose-limiting severe adverse effects. There is an unmet clinical need for targeted drug delivery systems that transport chemotherapeutics selectively to tumor cells while minimizing off-target toxicity. G protein-coupled receptors (GPCRs) are the largest family of membrane receptors, and many are overexpressed in solid tumors, including ovarian cancer. This review summarizes the progress in engineered nanoparticle research for drug delivery for ovarian cancer and discusses the potential use of GPCRs as molecular entry points to deliver anti-cancer compounds into ovarian cancer cells. A newly emerging treatment paradigm could be the personalized design of nanomedicines on a case-by-case basis.01A - Beitrag in wissenschaftlicher Zeitschrift
- PublikationValidation of a commercial enzyme-linked immunosorbent assay for allopregnanolone in the saliva of healthy pregnant women(MDPI, 27.09.2022) Grötsch, Maria Katharina; Wietor, Denise Margret; Hettich, Timm; Ehlert, Ulrike [in: Biomolecules]Enzyme-linked immunosorbent assays (ELISAs) for saliva are simple, non-invasive methods for hormone detection. Allopregnanolone (ALLO) is a neuroactive steroid hormone that plays a crucial role in the aetiology of reproductive mood disorders. To better understand the relationship between ALLO and mood, a validated method to measure peripheral hormone levels is required. Currently, there is no commercially available ELISA with which to measure ALLO in saliva. We validated two ELISAs, developed for use with blood, with the saliva samples of 25 pregnant women, examining the range and sensitivity, intra- and inter-assay precision, parallelism, linearity of dilution, and recovery. The samples were simultaneously analysed using the liquid-chromatography–mass-spectrometry (LC-MS) method. The kits differed in range (31.2–2000 pg/mL vs. 1.6–100 ng/mL) and sensitivity (<9.5 pg/mL vs. 0.9 ng/mL), with the latter showing significant matrix effects and the former fulfilling the acceptance criteria of all the parameters. The concentrations measured with LC–MS were below the lower limit of quantification (<1.0 ng/mL) and no signal was detected. One of the tested ELISAs is a valid method for detecting ALLO in the saliva of pregnant women. It has a suitable measurement range and higher sensitivity than the conventional LC–MS method.01A - Beitrag in wissenschaftlicher Zeitschrift
- PublikationWater-soluble optical sensors: keys to detect aluminium in biological environment(Royal Society of Chemistry, 01.01.2022) Unniram, Ajmal; Kavyashree, P.; Silswal, Akshay; Koner, Apurba Lal [in: RSC Advances]Metal ion plays a critical role from enzyme catalysis to cellular health and functions. The concentration of metal ions in a living system is highly regulated. Among the biologically relevant metal ions, the role and toxicity of aluminium in specific biological functions have been getting significant attention in recent years. The interaction of aluminium and the living system is unavoidable due to its high earth crust abundance, and the long-term exposure to aluminium can be fatal for life. The adverse Al3+ toxicity effects in humans result in various diseases ranging from cancers to neurogenetic disorders. Several Al3+ ions sensors have been developed over the past decades using the optical responses of synthesized molecules. However, only limited numbers of water-soluble optical sensors have been reported so far. In this review, we have confined our discussion to water-soluble Al3+ ions detection using optical methods and their utility for live-cell imaging and real-life application.01A - Beitrag in wissenschaftlicher Zeitschrift