Nanobiocatalysts with inbuilt cofactor recycling for oxidoreductase catalysis in organic solvents

dc.contributor.authorSahlin, Jenny
dc.contributor.authorWu, Congyu
dc.contributor.authorBuscemi, Andrea
dc.contributor.authorSchärer, Claude
dc.contributor.authorNazemi, Seyed Amirabbas
dc.contributor.authorS. K., Rejaul
dc.contributor.authorHerrera-Reinoza, Nataly
dc.contributor.authorJung, Thomas A.
dc.contributor.authorShahgaldian, Patrick
dc.date.accessioned2024-02-08T09:47:49Z
dc.date.available2024-02-08T09:47:49Z
dc.date.issued2023
dc.description.abstractThe major stumbling block in the implementation of oxidoreductase enzymes in continuous processes is their stark dependence on costly cofactors that are insoluble in organic solvents. We describe a chemical strategy that allows producing nanobiocatalysts, based on an oxidoreductase enzyme, that performs biocatalytic reactions in hydrophobic organic solvents without external cofactors. The chemical design relies on the use of a silica-based carrier nanoparticle, of which the porosity can be exploited to create an aqueous reservoir containing the cofactor. The nanoparticle core, possessing radial-centred pore channels, serves as a cofactor reservoir. It is further covered with a layer of reduced porosity. This layer serves as a support for the immobilisation of the selected enzyme yet allowing the diffusion of the cofactor from the nanoparticle core. The immobilised enzyme is, in turn, shielded by an organosilica layer of controlled thickness fully covering the enzyme. Such produced nanobiocatalysts are shown to catalyse the reduction of a series of relevant ketones into the corresponding secondary alcohols, also in a continuous flow fashion. © 2023 RSC.
dc.identifier.doi10.1039/d3na00413a
dc.identifier.issn2516-0230
dc.identifier.urihttps://irf.fhnw.ch/handle/11654/44044
dc.identifier.urihttps://doi.org/10.26041/fhnw-7939
dc.issue18
dc.language.isoen
dc.publisherRoyal Society of Chemistry
dc.relation.ispartofNanoscale Advances
dc.rights.urihttps://creativecommons.org/licenses/by-nc/4.0/
dc.subject.ddc500 - Naturwissenschaften und Mathematik
dc.titleNanobiocatalysts with inbuilt cofactor recycling for oxidoreductase catalysis in organic solvents
dc.type01A - Beitrag in wissenschaftlicher Zeitschrift
dc.volume5
dspace.entity.typePublication
fhnw.InventedHereYes
fhnw.ReviewTypeAnonymous ex ante peer review of a complete publication
fhnw.affiliation.hochschuleHochschule für Life Sciencesde_CH
fhnw.affiliation.institutInstitut für Chemie und Bioanalytikde_CH
fhnw.openAccessCategoryGold
fhnw.pagination5036-5044
fhnw.publicationStatePublished
relation.isAuthorOfPublication3482fed9-49e4-48ef-ae13-986df4e2a817
relation.isAuthorOfPublicationee2db471-989f-4135-8c86-c2b6a0e91eb0
relation.isAuthorOfPublication8884cd16-817b-4fba-a564-50a45970baa2
relation.isAuthorOfPublication.latestForDiscovery3482fed9-49e4-48ef-ae13-986df4e2a817
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