Microbiological landscape of oil-contaminated soil and its bioremediation by microorganisms
Author (Corporation)
Publication date
01.02.2024
Typ of student thesis
Course of study
Collections
Type
01A - Journal article
Editors
Editor (Corporation)
Supervisor
Parent work
Ecological Engineering & Environmental Technology
Special issue
DOI of the original publication
Link
Series
Series number
Volume
25
Issue / Number
2
Pages / Duration
31-40
Patent number
Publisher / Publishing institution
Polish Society of Ecological Engineering
Place of publication / Event location
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Abstract
The composition of microbial contaminants of soil samples contaminated with oil and oil products from oil depots of ports in southern Ukraine was investigated, and the possibility of their bioremediation by microorganisms present in the soil was determined. The microbiological landscape of the soil contaminated with oil and oil products was established, the quantitative and qualitative characteristics, group and dendrological composition of microorganisms were determined, and their potential ability to biodegrade petroleum hydrocarbons was determined. The degree of sanitary and ecological contamination of the samples was characterized by the number of the main groups of microorganisms - mesophilic aerobic and facultative anaerobic microorganisms (MAFAnM), molds, yeasts, as well as the dominance of MAFAnM by 3-5 orders among the studied groups of microorganisms. According to MAFANM, the number of thermophilic bacteria, titers of nitrifying bacteria, E. coli, Clostridium perfringens, bacteria of the genus Proteus, and the degree of oil contamination, the soil samples studied are characterized as contaminated and heavily contaminated. According to the study of morphological, tintorial, cultural, biochemical properties, 130 species were identified and 9 morphogroups of bacteria in oil-contaminated soil samples were determined. A dendrogram was constructed based on the set of studied properties of the isolated microorganisms. According to the results of the screening, the microorganisms isolated from contaminated soil samples are capable of biodegradation of long-chain alkanes of petroleum hydrocarbons. The identified groups of microorganisms can be arranged in the following sequence in order of increasing this indicator: Bacillus subtilis and Paenibacillus macerans ˂ Paenibacillus polymyxa ˂ Bacillus licheniformis ˂ Bacillus thuringiensis ˂ Bacillus megaterium ˂ Bacillus pumilis ˂ Bacillus cereus ˂ Paenibacillus circulans. Paenibacillus circulans and Bacillus cereus were identified as the most promising strains, biotransforming up to 48 percent of the total amount of hydrocarbons.
Keywords
Subject (DDC)
570 - Biowissenschaften, Biologie
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ISBN
ISSN
2719-7050
Language
English
Created during FHNW affiliation
Yes
Strategic action fields FHNW
Publication status
Published
Review
Peer review of the complete publication
Open access category
Gold
License
Citation
KUPRIYASHKINA, Helen, Liudmyla PYLYPENKO, Olena SEVASTYANOVA, Kseniya MAZURENKO, Christoph HUGI, Lena BREITENMOSER, Galyna KRUSIR und Myroslav MALYOVANYI, 2024. Microbiological landscape of oil-contaminated soil and its bioremediation by microorganisms. Ecological Engineering & Environmental Technology. 1 Februar 2024. Bd. 25, Nr. 2, S. 31–40. DOI 10.12912/27197050/175139. Verfügbar unter: https://doi.org/10.26041/fhnw-11711