Suter-Dick, Laura

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Suter-Dick
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Laura
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Suter-Dick, Laura

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2015 - 20193
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Autor:in: Weston, Anna × Institut: Institut für Chemie und Bioanalytik ×

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  • Vorschaubild nicht verfügbar
    Publikation
    Rat multicellular 3D liver microtissues to explore TGF-β1 induced effects
    (Elsevier, 13.11.2019) Prestigiacomo, Vincenzo; Weston, Anna; Suter-Dick, Laura [in: Journal of Pharmacological and Toxicological Methods]
    Chronic liver damage can lead to fibrosis, encompassing hepatocellular injury, activation of Kupffer cells (KC), and activation of hepatic stellate cells (HSC). Inflammation and TGF-β1 are known mediators in the liver fibrosis adverse outcome pathway (AOP). The aim of this project was to develop a suitable rodent cell culture model for the investigation of key events involved in the development of liver fibrosis, specifically the responses to pathophysiological stimuli such as TGF-β1 and LPS-triggered inflammation. We optimized a single step protocol to purify rat primary hepatocytes (Hep), HSC and KC cells to generate 3D co-cultures based on the hanging drop method. This primary multicellular model responded to the profibrotic cytokine TGF-β1 (1 ng/mL) with signs of hepatocellular damage, inflammation and ultimately HSC activation (increase in αSMA expression). LPS elicited an inflammatory response characterized by increased expression of cytokines. 3D-monocultures comprising only Hep displayed different responses, underlying that parenchymal and non-parenchymal cells need to be present in the system to recapitulate fibrosis. The data also suggest that pre-activated HSC may reverse to a quiescent phenotype in 3D, probably due to the more physiological conditions.
    01A - Beitrag in wissenschaftlicher Zeitschrift
  • Vorschaubild nicht verfügbar
    Publikation
    Modelling Alzheimer’s disease in three-dimensional human neural progenitor cultures
    (09/2018) Gaiser, Carine; Weston, Anna; Suter-Dick, Laura
    06 - Präsentation
  • Vorschaubild nicht verfügbar
    Publikation
    P12-040 High throughput in vitro system for nephrotoxicity testing
    (2015) Suter-Dick, Laura; Prétôt, René; Weston, Anna; Wegner, Irene; Wilmer, Martijn; Nieskens, Tom T.G.; Vulto, Paul; Joore, Jos; Lanz, Henriette; Masereeuw, Rosalinde
    06 - Präsentation