Grether, Yasmin

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Grether
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Yasmin
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Grether, Yasmin

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2016 - 20173
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Hochschule: Hochschule für Life Sciences ×

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  • Vorschaubild nicht verfügbar
    Publikation
    Altered (neo-) lacto series glycolipid biosynthesis impairs α2-6 sialylation on N-glycoproteins in ovarian cancer cells
    (Nature, 30.03.2017) Alam, Shahidul; Anugraham, Merrina; Huang, Yen-Lin; Kohler, Reto; Hettich, Timm; Winkelbach, Katharina; Grether, Yasmin; Nunez Lopez, Monica; Khasbiullina, Nailia; Bovin, Nicolai V.; Schlotterbeck, Götz; Jacob, Francis [in: Scientific Reports]
    The (neo-) lacto series glycosphingolipids (nsGSLs) comprise of glycan epitopes that are present as blood group antigens, act as primary receptors for human pathogens and are also increasingly associated with malignant diseases. Beta-1, 3-N-acetyl-glucosaminyl-transferase 5 (B3GNT5) is suggested as the key glycosyltransferase for the biosynthesis of nsGSLs. In this study, we investigated the impact of CRISPR-Cas9 -mediated gene disruption of B3GNT5 (∆B3GNT5) on the expression of glycosphingolipids and N-glycoproteins by utilizing immunostaining and glycomics-based PGC-UHPLC-ESI-QTOF-MS/MS profiling. ∆B3GNT5 cells lost nsGSL expression coinciding with reduction of α2-6 sialylation on N-glycoproteins. In contrast, disruption of B4GALNT1, a glycosyltransferase for ganglio series GSLs did not affect α2-6 sialylation on N-glycoproteins. We further profiled all known α2-6 sialyltransferase-encoding genes and showed that the loss of α2-6 sialylation is due to silencing of ST6GAL1 expression in ∆B3GNT5 cells. These results demonstrate that nsGSLs are part of a complex network affecting N-glycosylation in ovarian cancer cells.
    01A - Beitrag in wissenschaftlicher Zeitschrift
  • Vorschaubild nicht verfügbar
    Publikation
    Reduced Adhesion of E.coli on Nanostructured Polycarbonate Surfaces
    (08/2016) Grether, Yasmin; Waser, Marcus; Köser, Joachim
    06 - Präsentation
  • Vorschaubild nicht verfügbar
    Publikation
    Plasma-Etched Nanostructured Polycarbonate Surfaces for Reduced Adhesion of E.coli bacteria
    (30.06.2016) Grether, Yasmin; Waser, Marcus; Pieles, Uwe; Köser, Joachim
    Plasma-etching represents a facile approach to nanostructure polymer surfaces. Here we report on the antibacterial properties of plasma-structured polycarbonate (PC)surfaces. PC foils were structured with O2 plasma and incubated with E.coli. Metabolic activity of adhered live bacteria was quantified with resazurin metabolic indicator. Different incubation times under shaking/resting conditions diminished metabolic activity on structured versus unstructured PC, indicating reduced bacterial adhesion on structured surfaces. Furthermore chemical surface modifications increased metabolic activity on structured compared to unstructured surfaces. Further research on other polymers will be done to analyse the generality of these observations.
    06 - Präsentation