Berchtold, Christian

Berchtold, Christian


Gerade angezeigt 1 - 4 von 4
  • Publikation
    A UHPLC–MS/MS method for the quantification of 7α-hydroxy-4-cholesten-3-one to assist in diagnosis of bile acid malabsorption
    (Elsevier, 01/2017) Prost, Jean-Christophe; Brunner, Félix; Grob, Christian; Berchtold, Christian; Schlotterbeck, Götz; Bovet, Cédric; Largiadèr, Carlo R.; Fiedler, Georg Martin; Juillerat, Pascal [in: Clinical Mass Spectrometry]
    Bile acids malabsorption (BAM) is encountered in numerous gastrointestinal pathologies and is a good example of a treatable cause of watery diarrhea after ileal resection. The gold standard for diagnosing BAM is the selenium homocholic acid taurine test (SeHCAT), an expensive and complex analysis. An alternative method is the quantification of 7α-hydroxy-4-cholesten-3-one (C4). Here, we present a simple, ultra high-performance liquid chromatography–tandem mass spectrometry method to measure C4 in human serum. To avoid time consuming sample preparation (e.g., derivatization, solid phase extraction), we used absorption chemistry-based extraction plates. This method demonstrates a lower limit of quantification of 5 ng/mL and is linear over a concentration range from 5 to 300 ng/mL (R2 = 0.9977). Inaccuracy and imprecision were less than 15%. The validated method is currently used for routine measurement of C4 from serum in patients to confirm BAM diagnosis.
    01A - Beitrag in wissenschaftlicher Zeitschrift
  • Publikation
    Metabolic Profiling
    (2015) Berchtold, Christian; Hettich, Timm; Schlotterbeck, Götz; Senner, Frank [in: GIT: Labor-Fachzeitschrift]
    01 - Zeitschriftenartikel, Journalartikel oder Magazin
  • Publikation
    Validation of a simple and sensitive method for the quantification of the bile acid 7?-Hydroxy-4-cholesten-3-one (C4), (Poster)
    (2015) Berchtold, Christian; Schlotterbeck, Götz; Bovet, Cédric; Prost, Jean-Christophe; Grob, Christian
    06 - Präsentation
  • Publikation
    Minimizing carry-over for high throughput analysis
    (2015) Schlotterbeck, Götz; Berchtold, Christian; Böhm, Günter; Reto, Bolliger [in: International Symposium on High Performance Liquid Phase Separations (HPLC)]
    04 - Beitrag Sammelband oder Konferenzschrift