Berchtold, Christian

Lade...
Profilbild
E-Mail-Adresse
Geburtsdatum
Projekt
Organisationseinheiten
Berufsbeschreibung
Nachname
Berchtold
Vorname
Christian
Name
Berchtold, Christian

Suchergebnisse

Gerade angezeigt 1 - 4 von 4
Vorschaubild nicht verfügbar
Publikation

A UHPLC–MS/MS method for the quantification of 7α-hydroxy-4-cholesten-3-one to assist in diagnosis of bile acid malabsorption

2017-01, Prost, Jean-Christophe, Brunner, Félix, Grob, Christian, Berchtold, Christian, Schlotterbeck, Götz, Bovet, Cédric, Largiadèr, Carlo R., Fiedler, Georg Martin, Juillerat, Pascal

Bile acids malabsorption (BAM) is encountered in numerous gastrointestinal pathologies and is a good example of a treatable cause of watery diarrhea after ileal resection. The gold standard for diagnosing BAM is the selenium homocholic acid taurine test (SeHCAT), an expensive and complex analysis. An alternative method is the quantification of 7α-hydroxy-4-cholesten-3-one (C4). Here, we present a simple, ultra high-performance liquid chromatography–tandem mass spectrometry method to measure C4 in human serum. To avoid time consuming sample preparation (e.g., derivatization, solid phase extraction), we used absorption chemistry-based extraction plates. This method demonstrates a lower limit of quantification of 5 ng/mL and is linear over a concentration range from 5 to 300 ng/mL (R2 = 0.9977). Inaccuracy and imprecision were less than 15%. The validated method is currently used for routine measurement of C4 from serum in patients to confirm BAM diagnosis.

Vorschaubild nicht verfügbar
Publikation

Metabolic Profiling

2015, Berchtold, Christian, Hettich, Timm, Schlotterbeck, Götz, Senner, Frank

Vorschaubild nicht verfügbar
Publikation

Validation of a simple and sensitive method for the quantification of the bile acid 7?-Hydroxy-4-cholesten-3-one (C4), (Poster)

2015, Berchtold, Christian, Schlotterbeck, Götz, Bovet, Cédric, Prost, Jean-Christophe, Grob, Christian

Vorschaubild nicht verfügbar
Publikation

Minimizing carry-over for high throughput analysis

2015, Schlotterbeck, Götz, Berchtold, Christian, Böhm, Günter, Reto, Bolliger