Börnsen, Klaus Olaf

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Börnsen
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Klaus Olaf
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Klaus Olaf Börnsen

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    Assessment of the bony resection margin distance in bone-invasive oral cancer using laser-induced breakdown spectroscopy
    (Springer, 08.08.2024) Winnand, Philipp; Ooms, Mark; Heitzer, Marius; Vohl, Nils; Lammert, Matthias; Hölzle, Frank; Börnsen, Klaus Olaf; Modabber, Ali
    Objectives Inadequate resection margins of less than 5 mm impair local tumor control. This weak point in oncological safety is exacerbated in bone-infiltrating tumors because rapid bone analysis procedures do not exist. This study aims to assess the bony resection margin status of bone-invasive oral cancer using laser-induced breakdown spectroscopy (LIBS). Materials and methods LIBS experiments were performed on natively lasered, tumor-infiltrated mandibular cross-sections from 10 patients. In total, 5,336 spectra were recorded at defined distances from the tumor border. Resection margins < 1 mm were defined as very close, from 1–5 mm as close, and > 5 mm as clear. The spectra were histologically validated. Based on the LIBS spectra, the discriminatory power of potassium (K) and soluble calcium (Ca) between bone-infiltrating tumor tissue and very close, close, and clear resection margins was determined. Results LIBS-derived electrolyte emission values of K and soluble Ca as well as histological parameters for bone neogenesis/fibrosis and lymphocyte/macrophage infiltrates differ significantly between bone-infiltrating tumor tissue spectra and healthy bone spectra from very close, close, and clear resection margins (p < 0.0001). Using LIBS, the transition from very close resection margins to bone-infiltrating tumor tissue can be determined with a sensitivity of 95.0%, and the transition from clear to close resection margins can be determined with a sensitivity of 85.3%. Conclusions LIBS can reliably determine the boundary of bone-infiltrating tumors and might provide an orientation for determining a clear resection margin. Clinical relevance LIBS could facilitate intraoperative decision-making and avoid inadequate resection margins in bone-invasive oral cancer.
    01A - Beitrag in wissenschaftlicher Zeitschrift
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    Assessment of the electrolyte heterogeneity of tissues in mandibular bone-infiltrating head and neck cancer using laser-induced breakdown spectroscopy
    (MDPI, 23.02.2024) Winnand, Philipp; Börnsen, Klaus Olaf; Ooms, Mark; Heitzer, Marius; Vohl, Nils; Lammert, Matthias; Hölzle, Frank; Modabber, Ali
    Laser-induced breakdown spectroscopy (LIBS) was recently introduced as a rapid bone analysis technique in bone-infiltrating head and neck cancers. Research efforts on laser surgery systems with controlled tissue feedback are currently limited to animal specimens and the use of nontumorous tissues. Accordingly, this study aimed to characterize the electrolyte composition of tissues in human mandibular bone-infiltrating head and neck cancer. Mandible cross-sections from 12 patients with bone-invasive head and neck cancers were natively investigated with LIBS. Representative LIBS spectra (n = 3049) of the inferior alveolar nerve, fibrosis, tumor stroma, and cell-rich tumor areas were acquired and histologically validated. Tissue-specific differences in the LIBS spectra were determined by receiver operating characteristics analysis and visualized by principal component analysis. The electrolyte emission values of calcium (Ca) and potassium (K) significantly (p < 0.0001) differed in fibrosis, nerve tissue, tumor stroma, and cell-rich tumor areas. Based on the intracellular detection of Ca and K, LIBS ensures the discrimination between the inferior alveolar nerve and cell-rich tumor tissue with a sensitivity of ≥95.2% and a specificity of ≥87.2%. The heterogeneity of electrolyte emission values within tumorous and nontumorous tissue areas enables LIBS-based tissue recognition in mandibular bone-infiltrating head and neck cancer.
    01A - Beitrag in wissenschaftlicher Zeitschrift