Reduction-responsive immobilised and protected enzymes
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2025
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01A - Journal article
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Nanoscale Advances
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1
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Royal Society of Chemistry
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Abstract
We report a synthetic strategy to produce nano-immobilised and organosilica-shielded enzymes of which the biocatalytic activity is, by design, chemically enhanced under reductive conditions. The enzymes were immobilised onto silica nanoparticles through a reduction-responsive crosslinker and further shielded in an organosilica layer of controlled thickness. Under reducing conditions, disulphide bonds linking the protein to the carrier material were reduced, triggering enzyme activation. The organosilica shield prevents the enzymes from leaching from the nanobiocatalysts and preserves their integrity.
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2516-0230
Language
English
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Yes
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Published
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Gold
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Citation
Wu, C., Nazemi, A., Santacroce, N., Sahlin, J. A., Suter-Dick, L., & Shahgaldian, P. (2025). Reduction-responsive immobilised and protected enzymes. Nanoscale Advances, 1. https://doi.org/10.1039/d4na00580e