Method for analysis of electrospray for gene transfer and the impact on cell viability of A549 alveolar epithelial like cells
Loading...
Author (Corporation)
Publication date
2018
Typ of student thesis
Course of study
Type
04B - Conference paper
Editors
Editor (Corporation)
Supervisor
Parent work
2018 40th Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC)
Special issue
DOI of the original publication
Link
Series
Series number
Volume
Issue / Number
Pages / Duration
Patent number
Publisher / Publishing institution
IEEE
Place of publication / Event location
New York
Edition
Version
Programming language
Assignee
Practice partner / Client
Abstract
Electrospray is a process based on creation and
acceleration of small sized droplets based on electrostatic repulsion.
Spraying plasmid containing liquids this process may
be used to transfer genes into cells.
Within this paper we report on a method for accessing and
evaluating the spray modalities using high speed imaging system
with a post processing of image data to obtain estimated
volume and velocity of emerging droplets first. Second we investigate
on the impact of different media on the spray modalities.
Third we evaluate the impact of the spray on cell viability
and on transfection efficiency of an eGFP plasmid as reporter
gene obtained in an in vitro setup on alveolar epithelial like
cells (A549).
Keywords
Subject (DDC)
Event
40th Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC)
Exhibition start date
Exhibition end date
Conference start date
18.07.2018
Conference end date
21.07.2018
Date of the last check
ISBN
1558-4615
1557-170X
1557-170X
ISSN
Language
English
Created during FHNW affiliation
Yes
Strategic action fields FHNW
Publication status
Published
Review
Peer review of the complete publication
Open access category
Closed
License
Citation
Hradetzky, D., Boehringer, S., Ruzgys, P., Šatkauskas, S., Geiser, T., & Gazdhar, A. (2018). Method for analysis of electrospray for gene transfer and the impact on cell viability of A549 alveolar epithelial like cells. 2018 40th Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC). 40th Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC). https://doi.org/10.1109/embc.2018.8513643