Co‐current filtrate flow in TFF perfusion processes. Decoupling transmembrane pressure from crossflow to improve product sieving

Vorschaubild
Dateien
Biotech Bioengineering - 2023 - Romann - Co‐current filtrate flow in TFF perfusion processes Decoupling transmembrane.pdf(3.53 MB)
Autor:innen
Sibilia, Antony
Herwig, Christoph
Zydney, Andrew L.
Perilleux, Arnaud
Souquet, Jonathan
Bielser, Jean‐Marc
Autor:in (Körperschaft)
Publikationsdatum
2023
Typ der Arbeit
Studiengang
Sammlung
Institut für Pharma Technology
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Typ
01A - Beitrag in wissenschaftlicher Zeitschrift
Herausgeber:innen
Herausgeber:in (Körperschaft)
Betreuer:in
Übergeordnetes Werk
Biotechnology & Bioengineering
Themenheft
DOI der Originalpublikation
https://doi.org/10.1002/bit.28589
URI
https://irf.fhnw.ch/handle/11654/44018
https://doi.org/10.26041/fhnw-7916
Link
Reihe / Serie
Reihennummer
Jahrgang / Band
121
Ausgabe / Nummer
2
Seiten / Dauer
640-654
Patentnummer
Verlag / Herausgebende Institution
Wiley
Verlagsort / Veranstaltungsort
Auflage
Version
Programmiersprache
Abtretungsempfänger:in
Praxispartner:in/Auftraggeber:in
Zusammenfassung
Hollow fiber‐based membrane filtration has emerged as the dominant technology for cell retention in perfusion processes yet significant challenges in alleviating filter fouling remain unsolved. In this work, the benefits of co‐current filtrate flow applied to a tangential flow filtration (TFF) module to reduce or even completely remove Starling recirculation caused by the axial pressure drop within the module was studied by pressure characterization experiments and perfusion cell culture runs. Additionally, a novel concept to achieve alternating Starling flow within unidirectional TFF was investigated. Pressure profiles demonstrated that precise flow control can be achieved with both lab‐scale and manufacturing‐scale filters. TFF systems with co‐current flow showed up to 40% higher product sieving compared to standard TFF. The decoupling of transmembrane pressure from crossflow velocity and filter characteristics in co‐current TFF alleviates common challenges for hollow fiber‐based systems such as limited crossflow rates and relatively short filter module lengths, both of which are currently used to avoid extensive pressure drop along the filtration module. Therefore, co‐current filtrate flow in unidirectional TFF systems represents an interesting and scalable alternative to standard TFF or alternating TFF operation with additional possibilities to control Starling recirculation flow.
Schlagwörter
Starling recirculation, Perfusion cell culture, Tangential flow filtration (TFF)
Fachgebiet (DDC)
600 - Technik, Medizin, angewandte Wissenschaften
Projekt
Veranstaltung
Startdatum der Ausstellung
Enddatum der Ausstellung
Startdatum der Konferenz
Enddatum der Konferenz
Datum der letzten Prüfung
ISBN
ISSN
0006-3592
1097-0290
Sprache
Englisch
Während FHNW Zugehörigkeit erstellt
Ja
Publikationsstatus
Veröffentlicht
Begutachtung
Peer-Review der ganzen Publikation
Open Access-Status
Hybrid
Lizenz
'https://creativecommons.org/licenses/by/4.0/'
Zitation
ROMANN, Patrick, Philip GILLER, Antony SIBILIA, Christoph HERWIG, Andrew L. ZYDNEY, Arnaud PERILLEUX, Jonathan SOUQUET, Jean‐Marc BIELSER und Thomas VILLIGER, 2023. Co‐current filtrate flow in TFF perfusion processes. Decoupling transmembrane pressure from crossflow to improve product sieving. Biotechnology & Bioengineering. 2023. Bd. 121, Nr. 2, S. 640–654. DOI 10.1002/bit.28589. Verfügbar unter: https://doi.org/10.26041/fhnw-7916
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