Effects of hops derived prenylflavonoids on TNF-α induced barrier dysfunction in intestinal epithelial cells
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Autor:innen
Luescher, Sandro
Urmann, Corinna
Butterweck, Veronika
Autor:in (Körperschaft)
Publikationsdatum
2017
Typ der Arbeit
Studiengang
Sammlung
Typ
01A - Beitrag in wissenschaftlicher Zeitschrift
Herausgeber:innen
Herausgeber:in (Körperschaft)
Betreuer:in
Übergeordnetes Werk
Journal of Natural Products
Themenheft
DOI der Originalpublikation
Link
Reihe / Serie
Reihennummer
Jahrgang / Band
80
Ausgabe / Nummer
4
Seiten / Dauer
925-930
Patentnummer
Verlag / Herausgebende Institution
American Chemical Society
Verlagsort / Veranstaltungsort
Auflage
Version
Programmiersprache
Abtretungsempfänger:in
Praxispartner:in/Auftraggeber:in
Zusammenfassung
The human intestine allows the absorption of nutrients while also functioning as a barrier preventing pathogens from entering the mucosal tissues. Increased intestinal permeability is associated with autoimmune, inflammatory, and atopic diseases [1]. Tight junctions (TJ) are specialized junctional complexes forming a seal between adjacent epithelial cells.
Hops is a source of prenylflavonoids, including 6- and 8-prenylnaringenin (6-PN, 8-PN), xanthohumol (XN) and isoxanthohumol (IX) for which a variety of biological activities have been described.
The aims of this study were to a) establish a cell culture model for barrier dysfunction using TNF-α to induce TJ damage in Caco-2 cells; b) to test if hops-derived prenylflavonoids can prevent TNF-α induced TJ damage; and c) to investigate if 6-PN; 8-PN, XN, and IX can restore TNF-α induced barrier dysfunction.
After addition of TNF-α, the TEER value of Caco-2 cells demonstrated a significant decrease compared to the control group. From the tested compounds 6-PN and 8-PN prevented epithelial disruption induced by TNF-α, as assessed by measurement of TEER values. XN and IX also showed preventive effects, which occurred 60h after addition of TNF-α. Finally, it was of interest to determine possible treatment effects of XN, IX, 6-PN and 8-PN. Thus, TNF-α was added to Caco-2 cell monolayers for 24h before all test compounds were added. Under these experimental conditions only 8-PN significantly could antagonize TNF-α induced epithelial barrier dysfunction.
Our results show for the first time that 8-PN from hops attenuated a cytokine-induced increase in intestinal epithelial TJ permeability.
Schlagwörter
prenylflavonoids, hops, tight junctions, intestinal barrier, Caco-2 cells
Fachgebiet (DDC)
Veranstaltung
Startdatum der Ausstellung
Enddatum der Ausstellung
Startdatum der Konferenz
Enddatum der Konferenz
Datum der letzten Prüfung
ISBN
ISSN
0163-3864
1520-6025
1520-6025
Sprache
Englisch
Während FHNW Zugehörigkeit erstellt
Ja
Zukunftsfelder FHNW
Publikationsstatus
Veröffentlicht
Begutachtung
Peer-Review der ganzen Publikation
Open Access-Status
Lizenz
Zitation
LUESCHER, Sandro, Corinna URMANN und Veronika BUTTERWECK, 2017. Effects of hops derived prenylflavonoids on TNF-α induced barrier dysfunction in intestinal epithelial cells. Journal of Natural Products. 2017. Bd. 80, Nr. 4, S. 925–930. DOI 10.1055/s-0036-1596115. Verfügbar unter: http://hdl.handle.net/11654/25912