Saffron Flower Extract Promotes Scratch Wound Closure of Keratinocytes and Enhances VEGF Production
dc.accessRights | Anonymous | |
dc.audience | Science | |
dc.contributor.author | Verjee, Sheela | |
dc.contributor.author | Garo, Eliane | |
dc.contributor.author | Pelaez, Sarah | |
dc.contributor.author | Fertig, Orlando | |
dc.contributor.author | Hamburger, Matthias | |
dc.contributor.author | Butterweck, Veronika | |
dc.date.accessioned | 2018-01-09T12:57:47Z | |
dc.date.available | 2018-01-09T12:57:47Z | |
dc.date.issued | 2017 | |
dc.description.abstract | During saffron (Crocus sativus) spice production, large amounts of floral biowaste are generated. It was the aim of this study to develop a value-added product from saffron floral biowaste to be used as a natural cosmetic ingredient. HPLC-PDA-MS analysis of saffron flower extracts revealed the presence of flavonols with the highest amounts in the acetone extract. Kaempferol-3-O-sophoroside was identified as the main flavonoid in the acetone extract (saffron flower acetone extract). Saffron flower acetone extract and kaempferol-3-O-sophoroside were tested in HaCaT cells for potential effects on cell migration, proliferation, and for anti-inflammatory properties. Saffron flower acetone extract concentration dependently (50–200 µg/mL) augmented cell proliferation, as indicated by an increased BrdU-incorporation, while kaempferol-3-O-sophoroside (1–50 µM) had no effect. Furthermore, treatment of HaCaT cells with saffron flower acetone extract, but not with kaempferol-3-O-sophoroside, concentration-dependently increased vascular endothelial growth factor secretion (control 49.72 pg/mL vs. saffron flower acetone extract at 200 µg/mL 218.60 pg/mL). Cell migration was determined using time-lapse microscopy and a modification of the scratch-wound assay in which saffron flower acetone extract significantly improved wound closure compared to the untreated control. Overproduction of the proinflammatory cytokines interleukin-8 and interleukin-6 in HaCaT cells was induced by TNF-α. Kaempferol-3-O-sophoroside (10–50 µM), but not saffron flower acetone extract, inhibited TNF-α-induced IL-8 secretion. The effect was comparable to 10 µM hydrocortisone (positive control). Interestingly, saffron flower acetone extract further increased IL-6 levels in TNF-α-treated HaCaT cells in a concentration-dependent manner. In summary, the pronounced wound healing properties of saffron flower acetone extract present a promising application for the cosmetic industry. | |
dc.identifier.doi | 10.1055/s-0043-109002 | |
dc.identifier.issn | 1439-0221 | |
dc.identifier.issn | 0032-0943 | |
dc.identifier.uri | http://hdl.handle.net/11654/25774 | |
dc.issue | 14-15 | |
dc.language.iso | en | en_US |
dc.publisher | Thieme | en_US |
dc.relation.ispartof | Planta Medica | en_US |
dc.subject | Crocus sativus | |
dc.subject | biowaste | |
dc.subject | cell proliferation | |
dc.subject | cell migration | |
dc.subject | inflammation | |
dc.subject | kaempferol-3-O-sophoroside | |
dc.subject | cosmetics | |
dc.subject | Iridaceae | |
dc.title | Saffron Flower Extract Promotes Scratch Wound Closure of Keratinocytes and Enhances VEGF Production | |
dc.type | 01A - Beitrag in wissenschaftlicher Zeitschrift | |
dc.volume | 83 | |
dspace.entity.type | Publication | |
fhnw.InventedHere | Yes | |
fhnw.IsStudentsWork | no | |
fhnw.PublishedSwitzerland | Yes | |
fhnw.ReviewType | Anonymous ex ante peer review of a complete publication | |
fhnw.affiliation.hochschule | Hochschule für Life Sciences | de_CH |
fhnw.affiliation.institut | Institut für Pharma Technology | de_CH |
fhnw.pagination | 1176-1183 | |
fhnw.publicationOnline | Ja | |
fhnw.publicationState | Published | |
relation.isAuthorOfPublication | 169478d0-7102-45a6-9427-1d60caa2e5cf | |
relation.isAuthorOfPublication.latestForDiscovery | 169478d0-7102-45a6-9427-1d60caa2e5cf |