Parasite DNA detection in water samples enhances crayfish plague monitoring in asymptomatic invasive populations

dc.accessRightsAnonymous*
dc.contributor.authorSieber, Natalie
dc.contributor.authorHartikainen, Hanna
dc.contributor.authorKrieg, Raphael
dc.contributor.authorZenker, Armin
dc.contributor.authorVorburger, Christoph
dc.date.accessioned2022-03-28T07:45:59Z
dc.date.available2022-03-28T07:45:59Z
dc.date.issued2021-11-01
dc.description.abstractInvasive species can facilitate the spread of pathogens by first providing asymptomatic host reservoirs, and then driving disease outbreaks in native populations through pathogen spillover. An example of this are invasive crayfish species in Europe (Faxonius limosus, Pacifastacus leniusculus, Procambarus clarkii), which carry the deadly plague agent (Aphanomyces astaci). Effective disease management requires comprehensive monitoring, however, pathogen detection in carrier populations with low pathogen prevalence and intensities is challenging. We simultaneously collected and analysed crayfish tissue samples of invasive crayfish populations and water samples to compare A. astaci detection in different sample types using quantitative PCR. Combined, the two sampling methods revealed A. astaci presence with DNA concentrations above limit of detection (LOD; the lowest concentration which can be detected with reasonable certainty) in 13 of 23 invasive crayfish populations. In four additional sites, A. astaci DNA concentrations below LOD were found in water. In four populations only were A. astaci concentrations above LOD detected in both sample types and in three populations in concentrations above LOD in tissue but below LOD in water. The likely reason for these discrepancies is the low A. astaci prevalence and concentration in resistant invasive crayfish, which limit detection reliability. Consistency may be improved by timing surveys with seasonal periods of high A. astaci abundance and by increasing water sampling effort. Considering the ease of collecting eDNA samples, compared to crayfish tissue sampling, eDNA methods would facilitate frequent and comprehensive surveys. However, remaining uncertainties in eDNA-based detection reveal the relevance of combining monitoring tools to improve detection of invasive pathogens and their management.en_US
dc.identifier.doi10.1007/s10530-021-02644-y
dc.identifier.issn1387-3547
dc.identifier.issn1573-1464
dc.identifier.urihttps://irf.fhnw.ch/handle/11654/33399
dc.identifier.urihttps://doi.org/10.26041/fhnw-4147
dc.language.isoen_USen_US
dc.publisherSpringeren_US
dc.relation.ispartofBiological Invasionsen_US
dc.rightsAttribution 3.0 United States*
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/us/en_US
dc.subjectAphanomyces astacien_US
dc.subjectInvasive crayfishen_US
dc.subjectEnvironmental DNAen_US
dc.subjectPathogen monitoringen_US
dc.titleParasite DNA detection in water samples enhances crayfish plague monitoring in asymptomatic invasive populationsen_US
dc.type01A - Beitrag in wissenschaftlicher Zeitschrift
dc.volume24en_US
dspace.entity.typePublication
fhnw.InventedHereYesen_US
fhnw.IsStudentsWorknoen_US
fhnw.ReviewTypeAnonymous ex ante peer review of a complete publicationen_US
fhnw.affiliation.hochschuleHochschule für Life Sciences FHNWde_CH
fhnw.affiliation.institutInstitut für Ecopreneurshipde_CH
fhnw.openAccessCategoryHybriden_US
fhnw.pagination281-297en_US
fhnw.publicationStatePublisheden_US
relation.isAuthorOfPublicationc62f02ba-46c7-4b32-a82d-a72cae94e89f
relation.isAuthorOfPublication2c65ab8b-99bc-4a9f-9ec2-663290c42050
relation.isAuthorOfPublication.latestForDiscovery2c65ab8b-99bc-4a9f-9ec2-663290c42050
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