Enzyme Armoring by an Organosilica Layer: Synthesis and Characterization of Hybrid Organic/Inorganic Nanobiocatalysts

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Author (Corporation)
Publication date
02/2017
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01A - Journal article
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Parent work
Methods in Enzymology
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Volume
590
Issue / Number
Pages / Duration
77-91
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Academic Press
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Abstract
The availability of highly stable and reusable enzymes is one of the main challenges in bio-based industrial processes. Enzyme immobilization and encapsulation represent promising strategies to reach this goal. In this chapter, the synthetic strategy to produce hybrid organic/inorganic nanobiocatalysts (NBC) is reported. This strategy is based on the sequential immobilization of an enzyme on the surface of silica nanoparticles followed by the growth, at the surface of the nanoparticles, of a shielding layer which serves as an armor to protect the enzyme against denaturation/degradation. This armor is produced through a thickness-controlled organosilane poly-condensation onto the nanoparticle surface around the enzyme to form a protective organosilica layer. The armored nanobiocatalysts present enhanced catalytic activity and improved stability against heat, pH, chaotropic agents, proteases, and ultrasound. The method is versatile in that it can be successfully adapted to a number of different enzymes.
Keywords
Enzyme catalysis, Nanobiocatalysts, Nanoparticles, Organosilica, Self-assembly
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ISBN
ISSN
1557-7988
0076-6879
1079-2376
Language
English
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Yes
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Publication status
Published
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Peer review of the complete publication
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Citation
CORRERO, Maria Rita, Sabine SYKORA, Philippe CORVINI und Patrick SHAHGALDIAN, 2017. Enzyme Armoring by an Organosilica Layer: Synthesis and Characterization of Hybrid Organic/Inorganic Nanobiocatalysts. Methods in Enzymology. Februar 2017. Bd. 590, S. 77–91. DOI 10.1016/bs.mie.2016.12.006. Verfügbar unter: http://hdl.handle.net/11654/25904