Influence of salt type and ionic strength on self-assembly of dextran sulfate-ciprofloxacin nanoplexes

No Thumbnail Available
Authors
Kutscher, Marika
Cheow, Wean Sin
Werner, Vera
Lorenz, Udo
Ohlsen, Knut
Meinel, Lorenz
Hadinoto, Kunn
Author (Corporation)
Publication date
2015
Typ of student thesis
Course of study
Type
01A - Journal article
Editors
Editor (Corporation)
Supervisor
Parent work
International Journal of Pharmaceutics
Special issue
DOI of the original publication
Link
Series
Series number
Volume
486
Issue / Number
1-2
Pages / Duration
21-29
Patent number
Publisher / Publishing institution
Elsevier
Place of publication / Event location
Edition
Version
Programming language
Assignee
Practice partner / Client
Abstract
We evaluated an analytical setup to identify optimal preparation conditions for nanoplex formation of small molecule drugs and polyelectrolytes using ciprofloxacin (CIP) and dextran sulfate (DS) as model compounds. The suitability of isothermal titration calorimetry (ITC) as a screening tool for rational formulation optimization was assessed. Besides ITC, static and dynamic light scattering, zeta potential measurements and scanning electron microscopy were applied to analyze the influence of different salt types and ionic strengths on CIP/DS nanoplex formation. The addition of low amounts of salt, especially 0.1M NaCl, improved the formation of CIP/DS nanoplexes. The presence of low amounts of salt led to smaller and more numerous particles of higher uniformity but had no influence on the release of CIP from nanoplexes. Furthermore, the molar range, within which efficient complexation was achieved, was broader in the presence of 0.1M NaCl than in the absence of salt with overall comparable complexation efficiency. Importantly, binding affinity correlated with particle shape and morphology, potentially enabling optimization of critical quality attributes based on ITC data. Altogether, ITC along with supplemental methods is a versatile screening tool for the evaluation of nanoplex formulation conditions regarding mixing ratio, salt type and ionic strength.
Keywords
Ciprofloxacin, Dextran sulfate, Isothermal titration calorimetry, Polyelectrolyte complex
Subject (DDC)
Project
Event
Exhibition start date
Exhibition end date
Conference start date
Conference end date
Date of the last check
ISBN
ISSN
0378-5173
1873-3476
Language
English
Created during FHNW affiliation
Yes
Strategic action fields FHNW
Publication status
Published
Review
Peer review of the complete publication
Open access category
License
Citation
KUTSCHER, Marika, Wean Sin CHEOW, Vera WERNER, Udo LORENZ, Knut OHLSEN, Lorenz MEINEL, Kunn HADINOTO und Oliver GERMERSHAUS, 2015. Influence of salt type and ionic strength on self-assembly of dextran sulfate-ciprofloxacin nanoplexes. International Journal of Pharmaceutics. 2015. Bd. 486, Nr. 1-2, S. 21–29. DOI 10.1016/j.ijpharm.2015.03.022. Verfügbar unter: http://hdl.handle.net/11654/11963