A polyphenol-enriched fraction of rose oil distillation wastewater inhibits cell proliferation, migration and TNF-α-Induced VEGF secretion in human immortalized keratinocytes
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Authors
Wedler, Jonas
Rusanov, Krasimir
Atanassov, Ivan
Butterweck, Veronika
Author (Corporation)
Publication date
2016
Typ of student thesis
Course of study
Type
01A - Journal article
Editors
Editor (Corporation)
Supervisor
Parent work
Planta Medica
Special issue
DOI of the original publication
Link
Series
Series number
Volume
82
Issue / Number
11-12
Pages / Duration
1000-1008
Patent number
Publisher / Publishing institution
Thieme
Place of publication / Event location
Edition
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Programming language
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Practice partner / Client
Abstract
Water steam distillation of rose flowers separates the essential oil from the polyphenol-containing rose oil distillation wastewater. Recently, a strategy was developed to separate rose oil distillation wastewater into a polyphenol depleted water fraction and a polyphenol-enriched fraction [RF20-(SP-207)]. The objective of the present study was to investigate RF20-(SP-207) and fraction F(IV), augmented in quercetin and ellagic acid, for possible antiproliferative effects in immortalized human keratinocytes (HaCaT) since rose petals are known to contain compounds with potential antiproliferative activity.RF20-(SP-207) revealed dose-dependent antiproliferative activity (IC50 of 9.78 µg/mL). In a nontoxic concentration of 10 µg/mL, this effect was stronger than that of the two positive controls LY294002 (10 µM, PI3 K-inhibitor, 30 % inhibition) and NVP-BEZ235 (100 nM, dual PI3 K/mTOR inhibitor, 30 % inhibition) and clearly exceeded the antiproliferative action of quercetin (50 µM, 25 % inhibition) and ellagic acid (1 µM, 15 % inhibition). Time-lapse microscopy detected a significant impairment of cell migration of RF20-(SP-207) and F(IV). At concentrations of 10 µg/mL of both, extract and fraction, cell migration was strongly suppressed (51 % and 28 % gap closure, respectively, compared to 95 % gap closure 24 hours after control treatment). The suppression of cell migration was comparable to the positive controls LY294002, NVP-BEZ235, and quercetin. Furthermore, basal and TNF-α-stimulated VEGF-secretion was significantly reduced by RF20-(SP-207) and F(IV) at 10 µg/mL (44 % vs. untreated control).In conclusion, RF20-(SP-207) showed promising antiproliferative and antimigratory effects and could be developed as a supportive, therapy against hyperproliferation-involved skin diseases.
Keywords
Rosa damascene, wastewater, quercetin, Rosaceae, ellagic acid, cell proliferation, VEGF
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ISBN
ISSN
1439-0221
0032-0943
0032-0943
Language
English
Created during FHNW affiliation
Yes
Strategic action fields FHNW
Publication status
Published
Review
Peer review of the complete publication
Open access category
License
Citation
Wedler, J., Rusanov, K., Atanassov, I., & Butterweck, V. (2016). A polyphenol-enriched fraction of rose oil distillation wastewater inhibits cell proliferation, migration and TNF-α-Induced VEGF secretion in human immortalized keratinocytes. Planta Medica, 82(11-12), 1000–1008. https://doi.org/10.1055/s-0042-105158