A polyphenol-enriched fraction of rose oil distillation wastewater inhibits cell proliferation, migration and TNF-α-Induced VEGF secretion in human immortalized keratinocytes
dc.accessRights | Anonymous | |
dc.audience | Science | |
dc.contributor.author | Wedler, Jonas | |
dc.contributor.author | Rusanov, Krasimir | |
dc.contributor.author | Atanassov, Ivan | |
dc.contributor.author | Butterweck, Veronika | |
dc.date.accessioned | 2016-11-09T11:07:40Z | |
dc.date.available | 2016-11-09T11:07:40Z | |
dc.date.issued | 2016 | |
dc.description.abstract | Water steam distillation of rose flowers separates the essential oil from the polyphenol-containing rose oil distillation wastewater. Recently, a strategy was developed to separate rose oil distillation wastewater into a polyphenol depleted water fraction and a polyphenol-enriched fraction [RF20-(SP-207)]. The objective of the present study was to investigate RF20-(SP-207) and fraction F(IV), augmented in quercetin and ellagic acid, for possible antiproliferative effects in immortalized human keratinocytes (HaCaT) since rose petals are known to contain compounds with potential antiproliferative activity.RF20-(SP-207) revealed dose-dependent antiproliferative activity (IC50 of 9.78 µg/mL). In a nontoxic concentration of 10 µg/mL, this effect was stronger than that of the two positive controls LY294002 (10 µM, PI3 K-inhibitor, 30 % inhibition) and NVP-BEZ235 (100 nM, dual PI3 K/mTOR inhibitor, 30 % inhibition) and clearly exceeded the antiproliferative action of quercetin (50 µM, 25 % inhibition) and ellagic acid (1 µM, 15 % inhibition). Time-lapse microscopy detected a significant impairment of cell migration of RF20-(SP-207) and F(IV). At concentrations of 10 µg/mL of both, extract and fraction, cell migration was strongly suppressed (51 % and 28 % gap closure, respectively, compared to 95 % gap closure 24 hours after control treatment). The suppression of cell migration was comparable to the positive controls LY294002, NVP-BEZ235, and quercetin. Furthermore, basal and TNF-α-stimulated VEGF-secretion was significantly reduced by RF20-(SP-207) and F(IV) at 10 µg/mL (44 % vs. untreated control).In conclusion, RF20-(SP-207) showed promising antiproliferative and antimigratory effects and could be developed as a supportive, therapy against hyperproliferation-involved skin diseases. | |
dc.identifier.doi | 10.1055/s-0042-105158 | |
dc.identifier.issn | 1439-0221 | |
dc.identifier.issn | 0032-0943 | |
dc.identifier.uri | http://hdl.handle.net/11654/23427 | |
dc.issue | 11-12 | |
dc.language.iso | en | en_US |
dc.publisher | Thieme | en_US |
dc.relation.ispartof | Planta Medica | en_US |
dc.subject | Rosa damascene | |
dc.subject | wastewater | |
dc.subject | quercetin | |
dc.subject | Rosaceae | |
dc.subject | ellagic acid | |
dc.subject | cell proliferation | |
dc.subject | VEGF | |
dc.title | A polyphenol-enriched fraction of rose oil distillation wastewater inhibits cell proliferation, migration and TNF-α-Induced VEGF secretion in human immortalized keratinocytes | |
dc.type | 01A - Beitrag in wissenschaftlicher Zeitschrift | |
dc.volume | 82 | |
dspace.entity.type | Publication | |
fhnw.InventedHere | Yes | |
fhnw.IsStudentsWork | no | |
fhnw.PublishedSwitzerland | No | |
fhnw.ReviewType | Anonymous ex ante peer review of a complete publication | |
fhnw.affiliation.hochschule | Hochschule für Life Sciences | de_CH |
fhnw.affiliation.institut | Institut für Medizintechnik und Medizininformatik | de_CH |
fhnw.pagination | 1000-1008 | |
fhnw.publicationOnline | Ja | |
fhnw.publicationState | Published |
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