Comparison of the response of cultured osteoblasts and osteoblasts outgrown from rat calvarial bone chips to nonfouling KRSR and FHRRIKA‐peptide modified rough titanium surfaces
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Author (Corporation)
Publication date
11/2009
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Type
01A - Journal article
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Parent work
Journal of Biomedical Materials Research Part B: Applied Biomaterials
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Series
Series number
Volume
91B
Issue / Number
2
Pages / Duration
517 - 527
Patent number
Publisher / Publishing institution
Wiley
Place of publication / Event location
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Abstract
Mimicking proteins found in the extracellular matrix (ECM) using specific peptide sequences is a well-known strategy for the design of biomimetic surfaces, but has not yet been widely exploited in the field of biomedical implants. This study investigated osteoblast and, as a control, fibroblast proliferation to novel consensus heparin-binding peptides sequences KRSR and FHRIKKA that were immobilized onto rough (particle-blasted and chemically etched) commercially pure titanium surfaces using a poly(L-lysine)-graft- poly(ethylene glycol) (PLL-g-PEG) molecular assembly system. This platform enabled a detailed study of specific cell-peptide interactions even in the presence of serum in the culture medium; thanks to the excellent nonfouling properties of the PLL-g-PEG surface. Cell-binding peptide sequence RGD in combination with KRSR or FHRRIKA was used to examine a potentially-enhanced or synergistic effect on osteoblast proliferation. Bare titanium and bioinactive surfaces (i.e., unfunctionalized PLL-g-PEG and scrambled KSSR, RFHARIK, and RDG) were used as control substrates. Additionally, in a newly developed experimental setup, freshly harvested bone chips from newborn rat calvariae were placed onto the same type of surfaces investigating size and pattern of osteoblast outgrowths. The findings of the current study demonstrated that the difference in osteoblast and fibroblast proliferation was influenced by surface topography more so than by the presence of surface-bound KRSR and FHRRIKA. On the other hand, in comparison with the control surfaces, osteoblast outgrowths from rat calvarial bone chips covered a significantly larger area on RGD, KRSR, and FHRRIKA surfaces after 8 days and also migrated in an isotropic way unlike cells on the bioinactive substrates. Furthermore, the stimulatory effect of 0.75 pmol cm-2 RGD on osteoblast migration pattern could be enhanced when applied in combination with 2.25 pmol cm-2 KRSR.
Keywords
Biomimetic, Human gingival fibroblasts, Primary rat calvarial osteoblasts, Surface (bio)chemistry, Surface topography
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ISBN
ISSN
1552-4973
1552-4981
1552-4981
Language
English
Created during FHNW affiliation
No
Strategic action fields FHNW
Publication status
Published
Review
Peer review of the complete publication
Open access category
Closed
License
Citation
Schuler, M., Hamilton, D. W., Kunzler, T. P., Sprecher, C. M., de Wild, M., Brunette, D. M., Textor, M., & Tosatti, S. G. P. (2009). Comparison of the response of cultured osteoblasts and osteoblasts outgrown from rat calvarial bone chips to nonfouling KRSR and FHRRIKA‐peptide modified rough titanium surfaces. Journal of Biomedical Materials Research Part B: Applied Biomaterials, 91B(2), 517–527. https://doi.org/10.1002/jbm.b.31425