Comparison of the response of cultured osteoblasts and osteoblasts outgrown from rat calvarial bone chips to nonfouling KRSR and FHRRIKA‐peptide modified rough titanium surfaces
Vorschaubild nicht verfügbar
Autor:innen
Schuler, Martin
Hamilton, Douglas W.
Kunzler, Tobias P.
Sprecher, Christoph M.
Brunette, Donald M.
Textor, Marcus
Tosatti, Samuele G. P.
Autor:in (Körperschaft)
Publikationsdatum
11/2009
Typ der Arbeit
Studiengang
Typ
01A - Beitrag in wissenschaftlicher Zeitschrift
Herausgeber:innen
Herausgeber:in (Körperschaft)
Betreuer:in
Übergeordnetes Werk
Journal of Biomedical Materials Research Part B: Applied Biomaterials
Themenheft
DOI der Originalpublikation
Link
Reihe / Serie
Reihennummer
Jahrgang / Band
91B
Ausgabe / Nummer
2
Seiten / Dauer
517 - 527
Patentnummer
Verlag / Herausgebende Institution
Wiley
Verlagsort / Veranstaltungsort
Auflage
Version
Programmiersprache
Abtretungsempfänger:in
Praxispartner:in/Auftraggeber:in
Zusammenfassung
Mimicking proteins found in the extracellular matrix (ECM) using specific peptide sequences is a well-known strategy for the design of biomimetic surfaces, but has not yet been widely exploited in the field of biomedical implants. This study investigated osteoblast and, as a control, fibroblast proliferation to novel consensus heparin-binding peptides sequences KRSR and FHRIKKA that were immobilized onto rough (particle-blasted and chemically etched) commercially pure titanium surfaces using a poly(L-lysine)-graft- poly(ethylene glycol) (PLL-g-PEG) molecular assembly system. This platform enabled a detailed study of specific cell-peptide interactions even in the presence of serum in the culture medium; thanks to the excellent nonfouling properties of the PLL-g-PEG surface. Cell-binding peptide sequence RGD in combination with KRSR or FHRRIKA was used to examine a potentially-enhanced or synergistic effect on osteoblast proliferation. Bare titanium and bioinactive surfaces (i.e., unfunctionalized PLL-g-PEG and scrambled KSSR, RFHARIK, and RDG) were used as control substrates. Additionally, in a newly developed experimental setup, freshly harvested bone chips from newborn rat calvariae were placed onto the same type of surfaces investigating size and pattern of osteoblast outgrowths. The findings of the current study demonstrated that the difference in osteoblast and fibroblast proliferation was influenced by surface topography more so than by the presence of surface-bound KRSR and FHRRIKA. On the other hand, in comparison with the control surfaces, osteoblast outgrowths from rat calvarial bone chips covered a significantly larger area on RGD, KRSR, and FHRRIKA surfaces after 8 days and also migrated in an isotropic way unlike cells on the bioinactive substrates. Furthermore, the stimulatory effect of 0.75 pmol cm-2 RGD on osteoblast migration pattern could be enhanced when applied in combination with 2.25 pmol cm-2 KRSR.
Schlagwörter
Biomimetic, Human gingival fibroblasts, Primary rat calvarial osteoblasts, Surface (bio)chemistry, Surface topography
Fachgebiet (DDC)
600 - Technik, Medizin, angewandte Wissenschaften
Veranstaltung
Startdatum der Ausstellung
Enddatum der Ausstellung
Startdatum der Konferenz
Enddatum der Konferenz
Datum der letzten Prüfung
ISBN
ISSN
1552-4973
1552-4981
1552-4981
Sprache
Englisch
Während FHNW Zugehörigkeit erstellt
Nein
Publikationsstatus
Veröffentlicht
Begutachtung
Peer-Review der ganzen Publikation
Open Access-Status
Closed
Lizenz
Zitation
SCHULER, Martin, Douglas W. HAMILTON, Tobias P. KUNZLER, Christoph M. SPRECHER, Michael DE WILD, Donald M. BRUNETTE, Marcus TEXTOR und Samuele G. P. TOSATTI, 2009. Comparison of the response of cultured osteoblasts and osteoblasts outgrown from rat calvarial bone chips to nonfouling KRSR and FHRRIKA‐peptide modified rough titanium surfaces. Journal of Biomedical Materials Research Part B: Applied Biomaterials. November 2009. Bd. 91B, Nr. 2, S. 517 – 527. DOI 10.1002/jbm.b.31425. Verfügbar unter: https://irf.fhnw.ch/handle/11654/45807