Supramolecular enzyme engineering in complex nanometer-thin biomimetic organosilica layers
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Autor:innen
Autor:in (Körperschaft)
Publikationsdatum
09/2016
Typ der Arbeit
Studiengang
Typ
01A - Beitrag in wissenschaftlicher Zeitschrift
Herausgeber:innen
Herausgeber:in (Körperschaft)
Betreuer:in
Übergeordnetes Werk
RSC Advances
Themenheft
DOI der Originalpublikation
Link
Reihe / Serie
Reihennummer
Jahrgang / Band
6
Ausgabe / Nummer
92
Seiten / Dauer
89966-89971
Patentnummer
Verlag / Herausgebende Institution
Royal Society of Chemistry
Verlagsort / Veranstaltungsort
Auflage
Version
Programmiersprache
Abtretungsempfänger:in
Praxispartner:in/Auftraggeber:in
Zusammenfassung
The use of enzymes in industrial processes is often hampered by their limited stability under operational conditions. As enzymes' function and stability are directly correlated to their three-dimensional structure, numerous methods aiming at the preservation of this structure have been developed. While stabilization can be achieved using solid scaffolds for encapsulating the enzyme, it often results in loss of enzymic activity owing to a lack of conformational mobility of the biocatalyst. With the idea of mimicking protein-protein interactions to create a network of weak force interactions between the surface of an immobilized enzyme and a synthetic protective layer, we have developed a chem. strategy allowing the use of complex mixts. of building blocks mimicking the lateral chain of natural amino acids. After crosslinking a model enzyme at the surface of silica nanoparticles, incubation with eight different organosilane mixts. allowed growing protective organosilica layers of controlled thicknesses. The nanoparticles produced were characterized by SEM and their biocatalytic activity was measured under a series of operational stress conditions. Our results clearly demonstrated that increasing the complexity and biomimetic nature of the protection layer allowed for relevant improvement of the protection effect. Indeed, when compared with the basic formulation, selected complex formulations allowed for an improvement of up to 100% when treated at 50 °C for 60 min or in the presence of a denaturing detergent (SDS).
Schlagwörter
Fermentation, Bioindustrial Chemistry
Fachgebiet (DDC)
Veranstaltung
Startdatum der Ausstellung
Enddatum der Ausstellung
Startdatum der Konferenz
Enddatum der Konferenz
Datum der letzten Prüfung
ISBN
ISSN
2046-2069
Sprache
Englisch
Während FHNW Zugehörigkeit erstellt
Ja
Zukunftsfelder FHNW
Publikationsstatus
Veröffentlicht
Begutachtung
Peer-Review der ganzen Publikation
Open Access-Status
Lizenz
Zitation
CORRERO, Maria Rita, Michael TAKACS, Sabine SYKORA, Philippe CORVINI und Patrick SHAHGALDIAN, 2016. Supramolecular enzyme engineering in complex nanometer-thin biomimetic organosilica layers. RSC Advances. September 2016. Bd. 6, Nr. 92, S. 89966–89971. DOI 10.1039/C6RA17775A. Verfügbar unter: http://hdl.handle.net/11654/23396