Fabrication of TiO2‐coated epoxy replicas with identical dual‐type surface topographies used in cell culture assays
Loading...
Author (Corporation)
Publication date
01.2009
Typ of student thesis
Course of study
Type
01A - Journal article
Editors
Editor (Corporation)
Supervisor
Parent work
Journal of Biomedical Materials Research Part A
Special issue
DOI of the original publication
Link
Series
Series number
Volume
88A
Issue / Number
1
Pages / Duration
12 - 22
Patent number
Publisher / Publishing institution
Wiley
Place of publication / Event location
Edition
Version
Programming language
Assignee
Practice partner / Client
Abstract
The goal of this study was to reproducibly generate samples with complex surface topographies and chemistries identical to a "master surface" and to test their response in cell culture using rat calvarial cells. Negative replicas of dual-type topography were fabricated using dental impression material with half of the surface exhibiting smooth and rough topography, respectively. Positive epoxy resin replicas were cast from the same negative replica eight times consecutively and coated with a 60-nm thin film of titanium dioxide using a vapor deposition technique. Atomic force microscopy, scanning electron microscopy, confocal white light microscopy, and X-ray photoelectron spectroscopy indicated that TiO2-coated epoxy replicas had surface topographical features and surface compositions nearly indistinguishable from the original titanium master surfaces. The described technique showed high reproducibility over at least eight generations of replication using the same negative replica. Rat calvarial osteoblasts proliferated just as well on dual topography surfaces as on single topography surfaces. The advantage of the dual-type substrates is that they facilitate comparison within a single culture dish, thus eliminating dish-to-dish variation as well as saving material, time and costs compared to the usual method of evaluating surfaces in separate dishes.
Keywords
Cell morphology, Physical vapor deposition, Replica, Surface roughness, Titanium
Subject (DDC)
Event
Exhibition start date
Exhibition end date
Conference start date
Conference end date
Date of the last check
ISBN
ISSN
1549-3296
1552-4965
1552-4965
Language
English
Created during FHNW affiliation
No
Strategic action fields FHNW
Publication status
Published
Review
Peer review of the complete publication
Open access category
Closed
License
Citation
Schuler, M., Kunzler, T. P., de Wild, M., Sprecher, C. M., Trentin, D., Brunette, D. M., Textor, M., & Tosatti, S. G. P. (2009). Fabrication of TiO2‐coated epoxy replicas with identical dual‐type surface topographies used in cell culture assays. Journal of Biomedical Materials Research Part A, 88A(1), 12–22. https://doi.org/10.1002/jbm.a.31720