Fabrication of TiO2‐coated epoxy replicas with identical dual‐type surface topographies used in cell culture assays
Kein Vorschaubild vorhanden
Autor:in (Körperschaft)
Publikationsdatum
01/2009
Typ der Arbeit
Studiengang
Typ
01A - Beitrag in wissenschaftlicher Zeitschrift
Herausgeber:innen
Herausgeber:in (Körperschaft)
Betreuer:in
Übergeordnetes Werk
Journal of Biomedical Materials Research Part A
Themenheft
DOI der Originalpublikation
Link
Reihe / Serie
Reihennummer
Jahrgang / Band
88A
Ausgabe / Nummer
1
Seiten / Dauer
12 - 22
Patentnummer
Verlag / Herausgebende Institution
Wiley
Verlagsort / Veranstaltungsort
Auflage
Version
Programmiersprache
Abtretungsempfänger:in
Praxispartner:in/Auftraggeber:in
Zusammenfassung
The goal of this study was to reproducibly generate samples with complex surface topographies and chemistries identical to a "master surface" and to test their response in cell culture using rat calvarial cells. Negative replicas of dual-type topography were fabricated using dental impression material with half of the surface exhibiting smooth and rough topography, respectively. Positive epoxy resin replicas were cast from the same negative replica eight times consecutively and coated with a 60-nm thin film of titanium dioxide using a vapor deposition technique. Atomic force microscopy, scanning electron microscopy, confocal white light microscopy, and X-ray photoelectron spectroscopy indicated that TiO2-coated epoxy replicas had surface topographical features and surface compositions nearly indistinguishable from the original titanium master surfaces. The described technique showed high reproducibility over at least eight generations of replication using the same negative replica. Rat calvarial osteoblasts proliferated just as well on dual topography surfaces as on single topography surfaces. The advantage of the dual-type substrates is that they facilitate comparison within a single culture dish, thus eliminating dish-to-dish variation as well as saving material, time and costs compared to the usual method of evaluating surfaces in separate dishes.
Schlagwörter
Cell morphology, Physical vapor deposition, Replica, Surface roughness, Titanium
Fachgebiet (DDC)
600 - Technik, Medizin, angewandte Wissenschaften
Veranstaltung
Startdatum der Ausstellung
Enddatum der Ausstellung
Startdatum der Konferenz
Enddatum der Konferenz
Datum der letzten Prüfung
ISBN
ISSN
1549-3296
1552-4965
1552-4965
Sprache
Englisch
Während FHNW Zugehörigkeit erstellt
Nein
Zukunftsfelder FHNW
Publikationsstatus
Veröffentlicht
Begutachtung
Peer-Review der ganzen Publikation
Open Access-Status
Closed
Lizenz
Zitation
SCHULER, Martin, Tobias P. KUNZLER, Michael DE WILD, Christoph M. SPRECHER, Diana TRENTIN, Donald M. BRUNETTE, Marcus TEXTOR und Samuele G. P. TOSATTI, 2009. Fabrication of TiO2‐coated epoxy replicas with identical dual‐type surface topographies used in cell culture assays. Journal of Biomedical Materials Research Part A. Januar 2009. Bd. 88A, Nr. 1, S. 12 – 22. DOI 10.1002/jbm.a.31720. Verfügbar unter: https://irf.fhnw.ch/handle/11654/45808