Novel perfused compression bioreactor system as an in vitro model to investigate fracture healing
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2015
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01A - Journal article
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Frontiers in Bioengineering and Biotechnology
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3
Issue / Number
10
Pages / Duration
1-6
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Frontiers
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Abstract
Secondary bone fracture healing is a physiological process that leads to functional tissue
regeneration via endochondral bone formation. In vivo studies have demonstrated that
early mobilization and the application of mechanical loads enhances the process of fracture
healing. However, the influence of specific mechanical stimuli and particular effects during
specific phases of fracture healing remain to be elucidated. In this work, we have developed
and provided proof-of-concept of an in vitro human organotypic model of physiological
loading of a cartilage callus, based on a novel perfused compression bioreactor (PCB) system. We then used the fracture callus model to investigate the regulatory role of dynamic mechanical loading. Our findings provide a proof-of-principle that dynamic mechanical loading applied by the PCB can enhance the maturation process of mesenchymal stromal cells toward late hypertrophic chondrocytes and the mineralization of the deposited extracellular matrix. The PCB provides a promising tool to study fracture healing and for the in vitro assessment of alternative fracture treatments based on engineered tissue grafts or pharmaceutical compounds, allowing for the reduction of animal experiments.
Keywords
bioreactor, in vitro model, mechanical loading, hypertrophy, fracture healing
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2296-4185
Language
English
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Yes
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Published
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Citation
de Wild, M., Hoffmann, W., Feliciano, S., Martin, I., & Wendt, D. (2015). Novel perfused compression bioreactor system as an in vitro model to investigate fracture healing. Frontiers in Bioengineering and Biotechnology, 3(10), 1–6. https://doi.org/10.3389/fbioe.2015.00010