Novel perfused compression bioreactor system as an in vitro model to investigate fracture healing
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Autor:innen
Autor:in (Körperschaft)
Publikationsdatum
2015
Typ der Arbeit
Studiengang
Typ
01A - Beitrag in wissenschaftlicher Zeitschrift
Herausgeber:innen
Herausgeber:in (Körperschaft)
Betreuer:in
Übergeordnetes Werk
Frontiers in Bioengineering and Biotechnology
Themenheft
DOI der Originalpublikation
Link
Reihe / Serie
Reihennummer
Jahrgang / Band
3
Ausgabe / Nummer
10
Seiten / Dauer
1-6
Patentnummer
Verlag / Herausgebende Institution
Frontiers
Verlagsort / Veranstaltungsort
Auflage
Version
Programmiersprache
Abtretungsempfänger:in
Praxispartner:in/Auftraggeber:in
Zusammenfassung
Secondary bone fracture healing is a physiological process that leads to functional tissue
regeneration via endochondral bone formation. In vivo studies have demonstrated that
early mobilization and the application of mechanical loads enhances the process of fracture
healing. However, the influence of specific mechanical stimuli and particular effects during
specific phases of fracture healing remain to be elucidated. In this work, we have developed
and provided proof-of-concept of an in vitro human organotypic model of physiological
loading of a cartilage callus, based on a novel perfused compression bioreactor (PCB) system. We then used the fracture callus model to investigate the regulatory role of dynamic mechanical loading. Our findings provide a proof-of-principle that dynamic mechanical loading applied by the PCB can enhance the maturation process of mesenchymal stromal cells toward late hypertrophic chondrocytes and the mineralization of the deposited extracellular matrix. The PCB provides a promising tool to study fracture healing and for the in vitro assessment of alternative fracture treatments based on engineered tissue grafts or pharmaceutical compounds, allowing for the reduction of animal experiments.
Schlagwörter
bioreactor, in vitro model, mechanical loading, hypertrophy, fracture healing
Fachgebiet (DDC)
Veranstaltung
Startdatum der Ausstellung
Enddatum der Ausstellung
Startdatum der Konferenz
Enddatum der Konferenz
Datum der letzten Prüfung
ISBN
ISSN
2296-4185
Sprache
Englisch
Während FHNW Zugehörigkeit erstellt
Ja
Zukunftsfelder FHNW
Publikationsstatus
Veröffentlicht
Begutachtung
Peer-Review der ganzen Publikation
Open Access-Status
Lizenz
Zitation
de Wild, M., Hoffmann, W., Feliciano, S., Martin, I., & Wendt, D. (2015). Novel perfused compression bioreactor system as an in vitro model to investigate fracture healing. Frontiers in Bioengineering and Biotechnology, 3(10), 1–6. https://doi.org/10.3389/fbioe.2015.00010