Time Matters in Ecotoxicological Sampling Due to Circadian Rhythm

dc.accessRightsAnonymous
dc.audienceScience
dc.contributor.authorZhao, Yanbin
dc.contributor.authorFent, Karl
dc.date.accessioned2017-01-23T05:42:40Z
dc.date.available2017-01-23T05:42:40Z
dc.date.issued2016-03-24
dc.description.abstractAs in general, technological inventions also drive the development in the field of toxicology and ecotoxicology. In the past decade, gene expression analysis has become a universally applied technology allowing many insights into toxicological pathways of environmental contaminants. Due to the novel technologies, including quantitative determination of mRNA by quantitative reverse transcription analysis (qRT-PCR), and semiquantitative methods, such as microarrays and RNA-sequencing technologies, toxicological profiles of contaminants could be identified. For instance, gene expression analysis of genes associated with the hypothalamic-pituitary-gonadal axis (HPG axis) in fish had become a conventional end point for endocrine disrupting chemicals. While these gene expression data provide novel insights into identifying potential toxicological end points and molecular mechanisms, often not enough attention is given to the question of mRNA stabilities and reliabilities of transcriptional data, in particular when links to physiological effects are difficult to make. A crucial factor in this issue is the endogenous circadian oscillations of genes during sampling.
dc.identifier.doi10.1021/acs.est.6b01166
dc.identifier.issn1064-3389
dc.identifier.issn1547-6537
dc.identifier.urihttp://hdl.handle.net/11654/24032
dc.issue7
dc.language.isoenen
dc.publisherTaylor & Francisen_US
dc.relation.ispartofEnvironmental Science & Technologyen_US
dc.titleTime Matters in Ecotoxicological Sampling Due to Circadian Rhythm
dc.type01A - Beitrag in wissenschaftlicher Zeitschrift
dc.volume50
dspace.entity.typePublication
fhnw.InventedHereYes
fhnw.IsStudentsWorkno
fhnw.PublishedSwitzerlandNo
fhnw.ReviewTypeAnonymous ex ante peer review of a complete publication
fhnw.affiliation.hochschuleHochschule für Life Sciencesde_CH
fhnw.affiliation.institutInstitut für Ecopreneurshipde_CH
fhnw.pagination3287-3289
fhnw.publicationOnlineJa
fhnw.publicationStatePublished
relation.isAuthorOfPublication7e666602-999d-4a64-8e69-cffb29e30b4b
relation.isAuthorOfPublication.latestForDiscovery7e666602-999d-4a64-8e69-cffb29e30b4b
Dateien