Bryophyllum pinnatum inhibits oxytocin and vasopressin signaling in myometrial cells
Lade...
Autor:in (Körperschaft)
Publikationsdatum
10.04.2024
Typ der Arbeit
Studiengang
Sammlung
Typ
01A - Beitrag in wissenschaftlicher Zeitschrift
Herausgeber:innen
Herausgeber:in (Körperschaft)
Betreuer:in
Übergeordnetes Werk
Planta Medica
Themenheft
DOI der Originalpublikation
Link
Reihe / Serie
Reihennummer
Jahrgang / Band
90
Ausgabe / Nummer
10
Seiten / Dauer
757-765
Patentnummer
Verlag / Herausgebende Institution
Thieme
Verlagsort / Veranstaltungsort
Stuttgart
Auflage
Version
Programmiersprache
Abtretungsempfänger:in
Praxispartner:in/Auftraggeber:in
Zusammenfassung
The medicinal plant Bryophyllum pinnatum was previously shown to block oxytocin (OT)-induced signals in myometrial cells, consistent with its tocolytic effect observed in patients. OT activates not only OT receptors but also V 1A receptors, two receptors with high receptor homology that are both expressed in the myometrium and play a crucial role in myometrial contraction signaling. We aimed to study the molecular pharmacology of B. pinnatum herbal preparations using specific receptor ligands, the human myometrial cell line hTERT‑C3, and cell lines expressing recombinant human OT and V 1A receptors. We found that press juice from B. pinnatum (BPJ) inhibits both OT- and vasopressin (AVP)-induced intracellular calcium increases in hTERT‑C3 myometrial cells. In additional assays performed with cells expressing recombinant receptors, BPJ also inhibited OT and V 1A receptor-mediated signals with a similar potency (IC 50 about 0.5 mg/mL). We further studied endogenous OT- and AVP-sensitive receptors in hTERT‑C3 cells and found that OT and AVP stimulated those receptors with similar potency (EC50 of ~ 1 nM), suggesting expression of both receptor subtypes. This interpretation was corroborated by the antagonist potencies of atosiban and relcovaptan that we found. However, using qPCR, we almost exclusively found expression of OT receptors suggesting a pharmacological difference between recombinant OT receptors and native receptors expressed in hTERT‑C3 cells. In conclusion, we show that B. pinnatum inhibits both OT and AVP signaling, which may point beyond its tocolytic effects to other indications involving a disbalance in the vasopressinergic system.
Schlagwörter
Fachgebiet (DDC)
Veranstaltung
Startdatum der Ausstellung
Enddatum der Ausstellung
Startdatum der Konferenz
Enddatum der Konferenz
Datum der letzten Prüfung
ISBN
ISSN
0032-0943
1439-0221
1439-0221
Sprache
Englisch
Während FHNW Zugehörigkeit erstellt
Ja
Zukunftsfelder FHNW
Publikationsstatus
Veröffentlicht
Begutachtung
Peer-Review der ganzen Publikation
Open Access-Status
Closed
Lizenz
Zitation
Zurfluh, L., Duvaud, L., Inci, N., Potterat, O., Simões-Wüst, A. P., & Mosbacher, J. (2024). Bryophyllum pinnatum inhibits oxytocin and vasopressin signaling in myometrial cells. Planta Medica, 90(10), 757–765. https://doi.org/10.1055/a-2303-9608