Electrospray application for enchanced delivery of anticancer drugs into cells
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Autor:innen
Ruzgys, Paulius
Boehringer, Stephan
Tamò, Luca
Šatkauskas, Saulius
Geiser, Thomas
Gazdhar, Amiq
Autor:in (Körperschaft)
Publikationsdatum
2018
Typ der Arbeit
Studiengang
Typ
04B - Beitrag Konferenzschrift
Herausgeber:innen
Herausgeber:in (Körperschaft)
Betreuer:in
Übergeordnetes Werk
The vital nature sign. 12th international scientific conference, May 17-18, 2018, Kaunas, Lithuania. Abstract book.
Themenheft
DOI der Originalpublikation
Link
Reihe / Serie
Reihennummer
Jahrgang / Band
12
Ausgabe / Nummer
Seiten / Dauer
14
Patentnummer
Verlag / Herausgebende Institution
Vytautas Magnus university
Verlagsort / Veranstaltungsort
Kaunas
Auflage
Version
Programmiersprache
Abtretungsempfänger:in
Praxispartner:in/Auftraggeber:in
Zusammenfassung
Electrospray is a process based on applied high electric voltage on liquid, leading to the creation and acceleration of small sized droplets. Electrosprayed suspension with exogenous molecules on cells can trigger intracellular delivery. Here, the application study of anticancer drug delivery via electrospray as an antitumor treatment is presented in vitro and in vivo. In vitro experiments were performed on alveolar epithelial cells (A549). Cell viability was performed with flow cytometry assay. Apoptosis/ necrosis was measured using annexin V/PI kit. In vivo experiments were done with C57BL/6J mice. Lewis lung carcinoma (LLC) cell lines were injected intradermally to induce tumor. Electrospray was performed on tumor by cutting the skin. Tumor size was measured with caliper. Results show triggered PI transfer to cells after electrospray process. Moreover, obtained results in vitro showed successful cisplatin and methotrexate intracellular delivery, resulting in around 90 % of cell viability loss with methotrexate and 70 % of cell viability loss with cisplatin. In vivo experiments reveled a tumor decrease by around 2.5 times in 7 days after electrospray treatment at first and third day with both anticancer drugs. Here we show that electrospray method can be applied to local anticancer drug delivery to cells and tissues. Therefore, such method might be adapted as a clinical anticancer therapy in the future.
Schlagwörter
Electrospray, Drug Delivery, In Vitro, In Vivo
Fachgebiet (DDC)
Veranstaltung
12th international scientific conference
Startdatum der Ausstellung
Enddatum der Ausstellung
Startdatum der Konferenz
17.05.2018
Enddatum der Konferenz
18.05.2018
Datum der letzten Prüfung
ISBN
2335-8718
ISSN
Sprache
Englisch
Während FHNW Zugehörigkeit erstellt
Ja
Zukunftsfelder FHNW
Publikationsstatus
Veröffentlicht
Begutachtung
Peer-Review der ganzen Publikation
Open Access-Status
Closed
Lizenz
Zitation
RUZGYS, Paulius, Stephan BOEHRINGER, Luca TAMÒ, Saulius ŠATKAUSKAS, Thomas GEISER, Amiq GAZDHAR und David HRADETZKY, 2018. Electrospray application for enchanced delivery of anticancer drugs into cells. In: The vital nature sign. 12th international scientific conference, May 17-18, 2018, Kaunas, Lithuania. Abstract book. Kaunas: Vytautas Magnus university. 2018. S. 14. ISBN 2335-8718. Verfügbar unter: https://irf.fhnw.ch/handle/11654/46710