Electrospray application for enchanced delivery of anticancer drugs into cells
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Author (Corporation)
Publication date
2018
Typ of student thesis
Course of study
Type
04B - Conference paper
Editors
Editor (Corporation)
Supervisor
Parent work
The vital nature sign. 12th international scientific conference, May 17-18, 2018, Kaunas, Lithuania. Abstract book.
Special issue
DOI of the original publication
Link
Series
Series number
Volume
12
Issue / Number
Pages / Duration
14
Patent number
Publisher / Publishing institution
Vytautas Magnus university
Place of publication / Event location
Kaunas
Edition
Version
Programming language
Assignee
Practice partner / Client
Abstract
Electrospray is a process based on applied high electric voltage on liquid, leading to the creation and acceleration of small sized droplets. Electrosprayed suspension with exogenous molecules on cells can trigger intracellular delivery. Here, the application study of anticancer drug delivery via electrospray as an antitumor treatment is presented in vitro and in vivo. In vitro experiments were performed on alveolar epithelial cells (A549). Cell viability was performed with flow cytometry assay. Apoptosis/ necrosis was measured using annexin V/PI kit. In vivo experiments were done with C57BL/6J mice. Lewis lung carcinoma (LLC) cell lines were injected intradermally to induce tumor. Electrospray was performed on tumor by cutting the skin. Tumor size was measured with caliper. Results show triggered PI transfer to cells after electrospray process. Moreover, obtained results in vitro showed successful cisplatin and methotrexate intracellular delivery, resulting in around 90 % of cell viability loss with methotrexate and 70 % of cell viability loss with cisplatin. In vivo experiments reveled a tumor decrease by around 2.5 times in 7 days after electrospray treatment at first and third day with both anticancer drugs. Here we show that electrospray method can be applied to local anticancer drug delivery to cells and tissues. Therefore, such method might be adapted as a clinical anticancer therapy in the future.
Keywords
Electrospray, Drug Delivery, In Vitro, In Vivo
Subject (DDC)
Event
12th international scientific conference
Exhibition start date
Exhibition end date
Conference start date
17.05.2018
Conference end date
18.05.2018
Date of the last check
ISBN
2335-8718
ISSN
Language
English
Created during FHNW affiliation
Yes
Strategic action fields FHNW
Publication status
Published
Review
Peer review of the complete publication
Open access category
Closed
License
Citation
Ruzgys, P., Boehringer, S., Tamò, L., Šatkauskas, S., Geiser, T., Gazdhar, A., & Hradetzky, D. (2018). Electrospray application for enchanced delivery of anticancer drugs into cells. The Vital Nature Sign. 12th International Scientific Conference, May 17-18, 2018, Kaunas, Lithuania. Abstract Book., 12, 14. https://irf.fhnw.ch/handle/11654/46710