β‐D‐glucosidase assisted gold dissolution as non‐optical and quantifiable detection technique for immunoassays
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Author (Corporation)
Publication date
13.05.2013
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Type
01A - Journal article
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Editor (Corporation)
Supervisor
Parent work
Small
Special issue
DOI of the original publication
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Series
Series number
Volume
9
Issue / Number
23
Pages / Duration
4000-4005
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Publisher / Publishing institution
Wiley
Place of publication / Event location
Edition
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Abstract
Immunoassays are used for detecting protein targets for various applications. Here, a modification of immunoassays to allow a purely electrical detection of the target protein concentration is shown. The modification comprises a β-D-glucosidase as reporter enzyme and a cyanogenic glycoside as substrate. The enzymatic reaction produces cyanide in small quantities. For electrical detection of the cyanide, a novel sensor is developed, based on a gold micro wire. The cyanide dissolves the gold wire and changes the electrical resistance of the wire. Monitoring the resistance change allows a quantitative measurement of the target human C-reactive protein (an inflammatory marker) in blood plasma in the physiological relevant concentration range.
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Subject (DDC)
500 - Naturwissenschaften und Mathematik
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ISBN
ISSN
1613-6810
1613-6829
1613-6829
Language
English
Created during FHNW affiliation
No
Strategic action fields FHNW
Publication status
Published
Review
Peer review of the complete publication
Open access category
Closed
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Citation
KOEHLER, F. M., Renzo RASO, R. N. GRASS und W. J. STARK, 2013. β‐D‐glucosidase assisted gold dissolution as non‐optical and quantifiable detection technique for immunoassays. Small. 13 Mai 2013. Bd. 9, Nr. 23, S. 4000–4005. DOI 10.1002/smll.201300925. Verfügbar unter: https://irf.fhnw.ch/handle/11654/47283