β‐D‐glucosidase assisted gold dissolution as non‐optical and quantifiable detection technique for immunoassays
dc.contributor.author | Koehler, F. M. | |
dc.contributor.author | Raso, Renzo | |
dc.contributor.author | Grass, R. N. | |
dc.contributor.author | Stark, W. J. | |
dc.date.accessioned | 2024-09-12T14:33:23Z | |
dc.date.available | 2024-09-12T14:33:23Z | |
dc.date.issued | 2013-05-13 | |
dc.description.abstract | Immunoassays are used for detecting protein targets for various applications. Here, a modification of immunoassays to allow a purely electrical detection of the target protein concentration is shown. The modification comprises a β-D-glucosidase as reporter enzyme and a cyanogenic glycoside as substrate. The enzymatic reaction produces cyanide in small quantities. For electrical detection of the cyanide, a novel sensor is developed, based on a gold micro wire. The cyanide dissolves the gold wire and changes the electrical resistance of the wire. Monitoring the resistance change allows a quantitative measurement of the target human C-reactive protein (an inflammatory marker) in blood plasma in the physiological relevant concentration range. | |
dc.identifier.doi | 10.1002/smll.201300925 | |
dc.identifier.issn | 1613-6810 | |
dc.identifier.issn | 1613-6829 | |
dc.identifier.uri | https://irf.fhnw.ch/handle/11654/47283 | |
dc.issue | 23 | |
dc.language.iso | en | |
dc.publisher | Wiley | |
dc.relation.ispartof | Small | |
dc.subject.ddc | 500 - Naturwissenschaften und Mathematik | |
dc.title | β‐D‐glucosidase assisted gold dissolution as non‐optical and quantifiable detection technique for immunoassays | |
dc.type | 01A - Beitrag in wissenschaftlicher Zeitschrift | |
dc.volume | 9 | |
dspace.entity.type | Publication | |
fhnw.InventedHere | No | |
fhnw.ReviewType | Anonymous ex ante peer review of a complete publication | |
fhnw.affiliation.hochschule | Hochschule für Life Sciences FHNW | de_CH |
fhnw.affiliation.institut | Institut für Chemie und Bioanalytik | de_CH |
fhnw.openAccessCategory | Closed | |
fhnw.pagination | 4000-4005 | |
fhnw.publicationState | Published | |
relation.isAuthorOfPublication | 937e38aa-a894-4743-b5c5-053995a8b30e | |
relation.isAuthorOfPublication.latestForDiscovery | 937e38aa-a894-4743-b5c5-053995a8b30e |
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