β‐D‐glucosidase assisted gold dissolution as non‐optical and quantifiable detection technique for immunoassays

dc.contributor.authorKoehler, F. M.
dc.contributor.authorRaso, Renzo
dc.contributor.authorGrass, R. N.
dc.contributor.authorStark, W. J.
dc.date.accessioned2024-09-12T14:33:23Z
dc.date.available2024-09-12T14:33:23Z
dc.date.issued2013-05-13
dc.description.abstractImmunoassays are used for detecting protein targets for various applications. Here, a modification of immunoassays to allow a purely electrical detection of the target protein concentration is shown. The modification comprises a β-D-glucosidase as reporter enzyme and a cyanogenic glycoside as substrate. The enzymatic reaction produces cyanide in small quantities. For electrical detection of the cyanide, a novel sensor is developed, based on a gold micro wire. The cyanide dissolves the gold wire and changes the electrical resistance of the wire. Monitoring the resistance change allows a quantitative measurement of the target human C-reactive protein (an inflammatory marker) in blood plasma in the physiological relevant concentration range.
dc.identifier.doi10.1002/smll.201300925
dc.identifier.issn1613-6810
dc.identifier.issn1613-6829
dc.identifier.urihttps://irf.fhnw.ch/handle/11654/47283
dc.issue23
dc.language.isoen
dc.publisherWiley
dc.relation.ispartofSmall
dc.subject.ddc500 - Naturwissenschaften und Mathematik
dc.titleβ‐D‐glucosidase assisted gold dissolution as non‐optical and quantifiable detection technique for immunoassays
dc.type01A - Beitrag in wissenschaftlicher Zeitschrift
dc.volume9
dspace.entity.typePublication
fhnw.InventedHereNo
fhnw.ReviewTypeAnonymous ex ante peer review of a complete publication
fhnw.affiliation.hochschuleHochschule für Life Sciences FHNWde_CH
fhnw.affiliation.institutInstitut für Chemie und Bioanalytikde_CH
fhnw.openAccessCategoryClosed
fhnw.pagination4000-4005
fhnw.publicationStatePublished
relation.isAuthorOfPublication937e38aa-a894-4743-b5c5-053995a8b30e
relation.isAuthorOfPublication.latestForDiscovery937e38aa-a894-4743-b5c5-053995a8b30e
Dateien

Lizenzbündel

Gerade angezeigt 1 - 1 von 1
Kein Vorschaubild vorhanden
Name:
license.txt
Größe:
2.66 KB
Format:
Item-specific license agreed upon to submission
Beschreibung: