Surface modification of nanofibrous matrices via layer-by-layer functionalized silk assembly for mitigating the foreign body reaction
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Authors
Qian, Yuna
Li, Linhao
Song, Yang
Dong, Lili
Chen, Peixing
Li, Xiaoming
Cai, Kaiyong
Li, Yang
Fan, Yubo
Author (Corporation)
Publication date
05/2018
Typ of student thesis
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Type
01A - Journal article
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Supervisor
Parent work
Biomaterials
Special issue
DOI of the original publication
Link
Series
Series number
Volume
164
Issue / Number
Pages / Duration
22-37
Patent number
Publisher / Publishing institution
Elsevier
Place of publication / Event location
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Version
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Abstract
The inherent hydrophobicity and large surface area of electrospun synthetic polymeric scaffolds often cause non-specific protein adsorption, thereby influencing macrophage functions and eventually leading to fibrosis at the tissue-scaffold interface. This work reports fabrication of silk fibroin (SF)-functionalized electrospun polycaprolactone (PCL) fibers by single-component layer-by-layer assembly and decorate the SF with heparin disaccharide (HD), resulting in the non-covalent binding of interleukin-4 (IL-4) with the capacity to modulate macrophage polarization. A modified SF derivative was obtained by diazonium coupling and then covalently bonded with HD via click chemistry to eventually bind IL-4 efficiently and maintain its bioactivity. In vitro studies showed that IL-4 surface-functionalized nanofibrous scaffolds promoted polarization to M2 macrophages in the short-term. Importantly, in a murine subcutaneous implantation model, we found that promoting transient shifts in macrophage polarization at early stage can significantly inhibit the extent of the late foreign body reactions. Furthermore, the results of a transcriptomic profiling showed that MARK, PI3K, JNK and NF-κB signaling pathways played an important role in regulating the macrophage phenotypes in the SF/HD/IL-4-functionalized fibers. Our results suggest that such a strategy offers a new approach for utilizing biological agent surface functionalization to modulate the foreign body reaction to nanofibrous scaffolds.
Keywords
silk fibroin, electrospun, chick chemistry, macrophage, foreign body reaction
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ISBN
ISSN
0142-9612
1878-5905
1878-5905
Language
English
Created during FHNW affiliation
Yes
Strategic action fields FHNW
Publication status
Published
Review
Peer review of the complete publication
Open access category
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Citation
Qian, Y., Li, L., Song, Y., Dong, L., Chen, P., Li, X., Cai, K., Germershaus, O., Li, Y., & Fan, Y. (2018). Surface modification of nanofibrous matrices via layer-by-layer functionalized silk assembly for mitigating the foreign body reaction. Biomaterials, 164, 22–37. https://doi.org/10.1016/j.biomaterials.2018.02.038