Metabolite Profile and Antiproliferative Effects in HaCaT Cells of a Salix reticulata Extract
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Authors
Corradi, Elisabetta
Schmidt, Nadine
Räber, Nathalie
De Mieri, Maria
Hamburger, Matthias
Potterat, Oliver
Butterweck, Veronika
Author (Corporation)
Publication date
2017
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Type
01A - Journal article
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Parent work
Planta Medica
Special issue
DOI of the original publication
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Volume
83
Issue / Number
14/15
Pages / Duration
1149-1158
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Publisher / Publishing institution
Thieme
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Abstract
Phenolic constituents of Salix reticulata (Salicaceae) and antiproliferative activity of an extract and individual compounds were investigated in immortalized human non-tumorigenic keratinocytes (HaCaT). A MeOH extract from aerial parts afforded several flavonoids, including luteolin and apigenin glycosides (2–5 and 9) and catechin (1), two procyanidin fractions, and the phenolic glucosides picein (6), triandrin (7), and salicortin (8). In an adenosine triphosphate assay, the MeOH extract reduced cell viability by approximately 60 % at a concentration of 100 µg/mL. Cell proliferation was assessed with a BrdU incorporation ELISA assay. The extract inhibited proliferation of HaCaT cells in a concentration-dependent manner, with approximately 50 % inhibition at 100 µg/mL. In time-lapse assays, the extract showed distinct inhibitory effects on cell migration at concentrations of 12.5, 25, and 50 µg/mL. The activity of selected constituents was also determined. Luteolin-7-O-β-glucuronide (3) significantly inhibited cell proliferation at concentrations of 10 and 50 µM. In contrast, luteolin-7-O-β-glucopyranoside (2) and a procyanidin fraction (P1) had only weak effects, while picein (6) and salicortin (8) did not affect cell proliferation. Luteolin-7-O-β-glucuronide (10 µM) and, to a lesser extent, the procyanidin fraction (10 µg/mL) also inhibited cell migration.
Keywords
Salicaceae, procyanidins, phenolic glucosides, luteolin-7-O-β-glucuronide, antiproliferative activity, cell migration, Salix reticulata
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ISBN
ISSN
1439-0221
0032-0943
0032-0943
Language
English
Created during FHNW affiliation
Yes
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Publication status
Published
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Peer review of the complete publication
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Citation
CORRADI, Elisabetta, Nadine SCHMIDT, Nathalie RÄBER, Maria DE MIERI, Matthias HAMBURGER, Oliver POTTERAT und Veronika BUTTERWECK, 2017. Metabolite Profile and Antiproliferative Effects in HaCaT Cells of a Salix reticulata Extract. Planta Medica. 2017. Bd. 83, Nr. 14/15, S. 1149–1158. DOI 10.1055/s-0043-109098. Verfügbar unter: http://hdl.handle.net/11654/25794