A Biocatalytic Nanomaterial for the Label-Free Detection of Virus-Like Particles
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Authors
Belliot, Gaël
Pothier, Pierre
Dudal, Yves
Author (Corporation)
Publication date
2017
Typ of student thesis
Course of study
Collections
Type
01A - Journal article
Editors
Editor (Corporation)
Supervisor
Parent work
ChemBioChem
Special issue
DOI of the original publication
Link
Series
Series number
Volume
18
Issue / Number
11
Pages / Duration
996-1000
Patent number
Publisher / Publishing institution
Wiley
Place of publication / Event location
Edition
Version
Programming language
Assignee
Practice partner / Client
Abstract
The design of nanomaterials that are capable of specific and sensitive biomolecular recognition is an on-going challenge in the chemical and biochemical sciences. A number of sophisticated artificial systems have been designed to specifically recognize a variety of targets. However, methods based on natural biomolecular detection systems using antibodies are often superior. Besides greater affinity and selectivity, antibodies can be easily coupled to enzymatic systems that act as signal amplifiers, thus permitting impressively low detection limits. The possibility to translate this concept to artificial recognition systems remains limited due to design incompatibilities. Here we describe the synthesis of a synthetic nanomaterial capable of specific biomolecular detection by using an internal biocatalytic colorimetric detection and amplification system. The design of this nanomaterial relies on the ability to accurately grow hybrid protein-organosilica layers at the surface of silica nanoparticles. The method allows for label-free detection and quantification of targets at picomolar concentrations.
Keywords
Biocatalytic, nanomaterials, label-free detection, Virus-like Particles
Subject (DDC)
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ISBN
ISSN
1439-7633
1439-4227
1439-4227
Language
English
Created during FHNW affiliation
Yes
Strategic action fields FHNW
Publication status
Published
Review
Peer review of the complete publication
Open access category
License
Citation
Sykora, S., Correro, M. R., Moridi, N., Belliot, G., Pothier, P., Dudal, Y., Corvini, P., & Shahgaldian, P. (2017). A Biocatalytic Nanomaterial for the Label-Free Detection of Virus-Like Particles. ChemBioChem, 18(11), 996–1000. https://doi.org/10.1002/cbic.201700126