Sex blind. Bridging the gap between drug exposure and sex-related gene expression in Danio rerio using next-generation sequencing (NGS) data and a literature review to find the missing links in pharmaceutical and environmental toxicology studies

dc.contributor.authorKing, Alex C.
dc.contributor.authorZenker, Armin
dc.date.accessioned2023-09-08T09:14:01Z
dc.date.available2023-09-08T09:14:01Z
dc.date.issued2023-06-16
dc.description.abstract<jats:p>The sex of both humans and <jats:italic>Danio rerio</jats:italic> has previously been shown to affect the way individuals respond to drug exposure. Genes which allow identification of sex in juvenile zebrafish show potential to reveal these confounding variables between sex in toxicological and preclinical trials but the link between these is so far missing. These sex-specific, early expressed genes where expression is not altered by drug exposure must be carefully selected for this purpose. We aimed to discover genes which can be used in pharmaceutical trials and environmental toxicology studies to uncover sex-related variations in gene expression with drug application using the model organism <jats:italic>Danio rerio</jats:italic>. Previously published early sex determining genes from King et al. were evaluated as well as additional genes selected from our zebrafish Next-generation sequencing (NGS) data which are known from previously published works not to be susceptible to changes in expression with drug exposure. NGS revealed a further ten female-specific genes (vtg1, cyp17a1, cyp19a1a, igf3, ftz-f1, gdf9, foxl2a, Nr0b1, ipo4, lhcgr) and five male related candidate genes (FKBP5, apobb1, hbaa1, dmrt1, spata6) which are also expressed in juvenile zebrafish, 28 days post fertilisation (dpf). Following this, a literature review was performed to classify which of these early-expressed sex specific genes are already known to be affected by drug exposure in order to determine candidate genes to be used in pharmaceutical trials or environmental toxicology testing studies. Discovery of these early sex-determining genes in <jats:italic>Danio rerio</jats:italic> will allow identification of sex-related responses to drug testing to improve sex-specific healthcare and the medical treatment of human patients.</jats:p>
dc.identifier.doi10.3389/ftox.2023.1187302
dc.identifier.issn2673-3080
dc.identifier.urihttps://irf.fhnw.ch/handle/11654/37968
dc.identifier.urihttps://doi.org/10.26041/fhnw-5340
dc.language.isoen
dc.publisherFrontiers Media
dc.relation.ispartofFrontiers in Toxicology
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subject.ddc600 - Technik, Medizin, angewandte Wissenschaften
dc.titleSex blind. Bridging the gap between drug exposure and sex-related gene expression in Danio rerio using next-generation sequencing (NGS) data and a literature review to find the missing links in pharmaceutical and environmental toxicology studies
dc.type01A - Beitrag in wissenschaftlicher Zeitschrift
dc.volume5
dspace.entity.typePublication
fhnw.InventedHereYes
fhnw.ReviewTypeAnonymous ex ante peer review of a complete publication
fhnw.affiliation.hochschuleHochschule für Life Sciencesde_CH
fhnw.affiliation.institutInstitut für Ecopreneurshipde_CH
fhnw.openAccessCategoryGold
fhnw.publicationStatePublished
relation.isAuthorOfPublication2c65ab8b-99bc-4a9f-9ec2-663290c42050
relation.isAuthorOfPublication.latestForDiscovery2c65ab8b-99bc-4a9f-9ec2-663290c42050
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