Photopolymerization of L-DOPA-alginate immobilized cell wall laccase for textile dye decolorization
Author (Corporation)
Publication date
01.06.2024
Typ of student thesis
Course of study
Collections
Type
01A - Journal article
Editors
Editor (Corporation)
Supervisor
Parent work
Biomass
Special issue
DOI of the original publication
Link
Series
Series number
Volume
4
Issue / Number
2
Pages / Duration
490-506
Patent number
Publisher / Publishing institution
MDPI
Place of publication / Event location
Edition
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Abstract
Alginate is a naturally occurring polymer derived from brown algae biomass, which has numerous applications in various fields. Chemical modification of alginate is widely used to improve alginate’s physicochemical properties and provide new potential for multiple applications. In this article, we modified alginate with L-DOPA, using periodate oxidation and reductive amination, to obtain more suitable biopolymer for biocatalyst immobilization and hydrogel formation. Obtained modified alginate was used for the immobilization of laccase on cell walls. For this purpose, laccase from Streptomyces cyaneus was expressed on the surface of Saccharomyces cerevisiae EBY100 cells. The obtained cell wall laccase was immobilized within L-DOPA-alginate beads by crosslinking the L-DOPA-alginate with calcium ions and laccase. The effect of additional crosslinking of beads by green light-induced photopolymerization with eosin Y was investigated. The immobilized laccase systems were used for dye decolorization and investigated in multiple treatment processes. Beads with L-DOPA-alginate with a higher degree of modification (5.0 mol%) showed higher enzymatic activity and better decolorization efficiency than those with a lower degree of modification (2.5 mol%). Obtained immobilized biocatalysts are suitable for decolorizing dye Evans Blue due to their high efficiency and reusability.
Keywords
Subject (DDC)
600 - Technik, Medizin, angewandte Wissenschaften
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ISBN
ISSN
2673-8783
Language
English
Created during FHNW affiliation
Yes
Strategic action fields FHNW
Publication status
Published
Review
Peer review of the complete publication
Open access category
Gold
License
Citation
KOKAR, Nikolina Popović, Anamarija NIKOLETIC, Marija STANIŠIĆ, Milica CRNOGLAVAC POPOVIĆ und Radivoje PRODANOVIĆ, 2024. Photopolymerization of L-DOPA-alginate immobilized cell wall laccase for textile dye decolorization. Biomass. 1 Juni 2024. Bd. 4, Nr. 2, S. 490–506. DOI 10.3390/biomass4020025. Verfügbar unter: https://doi.org/10.26041/fhnw-11915