A new electrospray method for targeted gene delivery
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Authors
Boehringer, Stephan
Ruzgys, Paulius
Tamò, Luca
Satkauskas, Saulius
Geiser, Thomas
Gazdhar, Amiq
Author (Corporation)
Publication date
03/2018
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Type
01A - Journal article
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Parent work
Scientific Reports
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DOI of the original publication
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Volume
8
Issue / Number
1
Pages / Duration
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Publisher / Publishing institution
Nature
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Abstract
A challenge for gene therapy is absence of safe and efficient local delivery of therapeutic genetic material. An efficient and reproducible physical method of electrospray for localized and targeted gene delivery is presented. Electrospray works on the principle of coulombs repulsion, under influence of electric field the liquid carrying genetic material is dispersed into micro droplets and is accelerated towards the targeted tissue, acting as a counter electrode. The accelerated droplets penetrate the targeted cells thus facilitating the transfer of genetic material into the cell. The work described here presents the principle of electrospray for gene delivery, the basic instrument design, and the various optimized parameters to enhance gene transfer in vitro. We estimate a transfection efficiency of up to 60% was achieved. We describe an efficient gene transfer method and a potential electrospray-mediated gene transfer mechanism.
Keywords
electrospray, targeted gene delivery, coulombs repulsion, Electric field
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ISSN
2045-2322
Language
English
Created during FHNW affiliation
Yes
Strategic action fields FHNW
Publication status
Published
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Peer review of the complete publication
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Citation
Boehringer, S., Ruzgys, P., Tamò, L., Satkauskas, S., Geiser, T., Gazdhar, A., & Hradetzky, D. (2018). A new electrospray method for targeted gene delivery. Scientific Reports, 8(1). https://doi.org/10.1038/s41598-018-22280-2