Saponins from saffron corms inhibit the gene expression and secretion of pro-inflammatory cytokines
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Authors
Keller, Morris
Fankhauser, Sarah
Giezendanner, Noreen
König, Michelle
Keresztes, Franziska
Danton, Ombeline
Fertig, Orlando
Marcourt, Laurence
Butterweck, Veronika
Potterat, Olivier
Author (Corporation)
Publication date
18.02.2021
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01A - Journal article
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Parent work
Natural Products
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Volume
84
Issue / Number
3
Pages / Duration
630-645
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Publisher / Publishing institution
American Chemical Society
Place of publication / Event location
Washington
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Abstract
Corms are obtained as a byproduct during the cultivation of saffron (Crocus sativus). In a project aimed at the valorization of this waste product, we observed that a 70% EtOH extract of the corms and a sugar-depleted MeOH fraction of the extract inhibited the TNF-α/IFN-γ-induced secretion and gene expression of the chemokines IL-8, MCP-1, and RANTES in human HaCaT cells. The effects were in part stronger than those of the positive control hydrocortisone. For preparative isolation, the 70% EtOH extract was partitioned between n-BuOH and water. Separation of the n-BuOH-soluble fraction by centrifugal partition chromatography, followed by preparative and semipreparative HPLC, afforded a series of bidesmosidic glycosides of echinocystic acid bearing a 3,16-dihydroxy-10-oxo-hexadecanoic acid residue attached to the glycosidic moiety at C-28. They include azafrines 1 and 2, previously reported in saffron, and eight new congeners named azafrines 3–10. Saffron saponins significantly inhibited TNF-α/IFN-γ-induced secretion of RANTES in human HaCaT cells at 1 μM (p < 0.001). Some of them further lowered TNF-α/IFN-γ-induced gene expression.
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ISBN
ISSN
0163-3864
1520-6025
1520-6025
Language
English
Created during FHNW affiliation
Yes
Strategic action fields FHNW
Publication status
Published
Review
Peer review of the complete publication
Open access category
Closed
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Citation
Keller, M., Fankhauser, S., Giezendanner, N., König, M., Keresztes, F., Danton, O., Fertig, O., Marcourt, L., Butterweck, V., Potterat, O., & Hamburger, M. (2021). Saponins from saffron corms inhibit the gene expression and secretion of pro-inflammatory cytokines. Natural Products, 84(3), 630–645. https://doi.org/10.1021/acs.jnatprod.0c01220