Directional submicrofiber hydrogel composite scaffolds supporting neuron differentiation and enabling neurite alignment
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Authors
Selvi, Jasmin
Faia-Torres, Ana Bela
Rühe, Jürgen
Author (Corporation)
Publication date
29.09.2022
Typ of student thesis
Course of study
Type
01A - Journal article
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Editor (Corporation)
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Parent work
International Journal of Molecular Sciences
Special issue
DOI of the original publication
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Series
Series number
Volume
23
Issue / Number
19
Pages / Duration
Patent number
Publisher / Publishing institution
MDPI
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Abstract
Cell cultures aiming at tissue regeneration benefit from scaffolds with physiologically relevant elastic moduli to optimally trigger cell attachment, proliferation and promote differentiation, guidance and tissue maturation. Complex scaffolds designed with guiding cues can mimic the anisotropic nature of neural tissues, such as spinal cord or brain, and recall the ability of human neural progenitor cells to differentiate and align. This work introduces a cost-efficient gelatin-based submicron patterned hydrogel–fiber composite with tuned stiffness, able to support cell attachment, differentiation and alignment of neurons derived from human progenitor cells. The enzymatically crosslinked gelatin-based hydrogels were generated with stiffnesses from 8 to 80 kPa, onto which poly(ε-caprolactone) (PCL) alignment cues were electrospun such that the fibers had a preferential alignment. The fiber–hydrogel composites with a modulus of about 20 kPa showed the strongest cell attachment and highest cell proliferation, rendering them an ideal differentiation support. Differentiated neurons aligned and bundled their neurites along the aligned PCL filaments, which is unique to this cell type on a fiber–hydrogel composite. This novel scaffold relies on robust and inexpensive technology and is suitable for neural tissue engineering where directional neuron alignment is required, such as in the spinal cord.
Keywords
Electrospinning, Fiber-hydrogel scaffold, Neural cell guiding, Neurite alignment
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ISBN
ISSN
1422-0067
1661-6596
1661-6596
Language
English
Created during FHNW affiliation
Yes
Strategic action fields FHNW
Publication status
Published
Review
Peer review of the complete publication
Open access category
Gold
License
Citation
Selvi, J., Faia-Torres, A. B., Rühe, J., Züger, F., Suter-Dick, L., Mungenast, L., & Gullo, M. (2022). Directional submicrofiber hydrogel composite scaffolds supporting neuron differentiation and enabling neurite alignment. International Journal of Molecular Sciences, 23(19). https://doi.org/10.3390/ijms231911525