An artificial metalloenzyme for carbene transfer based on a biotinylated dirhodium anchored within streptavidin

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Authors
Zhao, Jingming
Bachmann, Daniel G.
Gillingham, Dennis G.
Ward, Thomas R.
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Publication date
04/2018
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01A - Journal article
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Parent work
Catalysis Science & Technology
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Volume
2018
Issue / Number
8
Pages / Duration
2294-2298
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Royal Society of Chemistry
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Abstract
We report on artificial metalloenzymes that incorporate a biotinylated dirhodium core embedded within engineered streptavidin (Sav hereafter) variants. The resulting biohybrid catalyzes the carbene insertion in C–H bonds and olefins. Chemical- and genetic optimization allows to modulate the catalytic activity of the artificial metalloenzymes that are shown to be active in the periplasm of E. coli (up to 20 turnovers).
Keywords
metalloenzymes, biotinylated dirhodium core, streptavidin
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2044-4761
2044-4753
Language
English
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Yes
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Published
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Peer review of the complete publication
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Citation
ZHAO, Jingming, Daniel G. BACHMANN, Markus LENZ, Dennis G. GILLINGHAM und Thomas R. WARD, 2018. An artificial metalloenzyme for carbene transfer based on a biotinylated dirhodium anchored within streptavidin. Catalysis Science & Technology. April 2018. Bd. 2018, Nr. 8, S. 2294–2298. DOI 10.1039/C8CY00646F. Verfügbar unter: http://hdl.handle.net/11654/27038