Evaluation of calcium dihydroxide- and silver-coated implants in the rat tibia

Loading...
Thumbnail Image
Authors
Harrasser, Norbert
Gorkotte, Johannes
Obermeier, Andreas
Feihl, Susanne
Author (Corporation)
Publication date
02.11.2016
Typ of student thesis
Course of study
Type
01A - Journal article
Editors
Editor (Corporation)
Supervisor
Parent work
Journal of Applied Biomaterials & Functional Materials
Special issue
DOI of the original publication
Link
Series
Series number
Volume
14
Issue / Number
4
Pages / Duration
e441-e448
Patent number
Publisher / Publishing institution
SAGE
Place of publication / Event location
Edition
Version
Programming language
Assignee
Practice partner / Client
Abstract
Silver ions (Ag+) have strong antibacterial effects, and silver-coated materials are in widespread clinical use. However, the application of silver-coated medical devices is not without concerns: its use with direct bone contact is not established, and systemic toxic side effects of released Ag+ have been described. Therefore, alternative bactericidal coatings with a more localized way of acting - e.g., calcium dihydroxide, Ca(OH)2 (CH) - would be advantageous. A new rat model of the animal's tibial metaphysis was developed. In the left proximal tibiae of 36 male Wistar rats, titanium screws were implanted. The screws were coated with hydroxyapatite (HA; 12 animals: group I), low-dosed HA silver (HA-Ag; 12 animals: group II) and CH (12 animals: group III). After 6 weeks, all rats were sacrificed. The implants were evaluated for morphological changes on their surfaces, by light microscopy, scanning electron microscopy and energy-dispersive X-ray spectroscopy; for osteointegration, by measurement of resistance to removal; and for bacterial colonization, by quantitative culture analysis. Additionally, the tibial bone was investigated histologically for signs of osteomyelitis and sonicated to detect bacterial loads. (i) No microbiological or histological signs of infection could be determined on any of the screws or the surrounding bone. (ii) The bone-implant interface analysis revealed extensive bone formation and direct bone-implant contact on all HA, HA-Ag and HA-CH coated screws. (iii) HA and HA-Ag were partially, and CH was fully, degraded on the screw coating, allowing host bone to osteointegrate.
Keywords
Subject (DDC)
Project
Event
Exhibition start date
Exhibition end date
Conference start date
Conference end date
Date of the last check
ISBN
ISSN
2280-0800
2280-8000
Language
English
Created during FHNW affiliation
Yes
Strategic action fields FHNW
Publication status
Published
Review
Peer review of the complete publication
Open access category
License
Citation
Harrasser, N., de Wild, M., Gorkotte, J., Obermeier, A., & Feihl, S. (2016). Evaluation of calcium dihydroxide- and silver-coated implants in the rat tibia. Journal of Applied Biomaterials & Functional Materials, 14(4), e441–e448. https://doi.org/10.5301/jabfm.5000323