Validation of a commercial enzyme-linked immunosorbent assay for allopregnanolone in the saliva of healthy pregnant women
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Publication date
27.09.2022
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01A - Journal article
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Parent work
Biomolecules
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Volume
12
Issue / Number
10
Pages / Duration
1-10
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MDPI
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Abstract
Enzyme-linked immunosorbent assays (ELISAs) for saliva are simple, non-invasive methods for hormone detection. Allopregnanolone (ALLO) is a neuroactive steroid hormone that plays a crucial role in the aetiology of reproductive mood disorders. To better understand the relationship between ALLO and mood, a validated method to measure peripheral hormone levels is required. Currently, there is no commercially available ELISA with which to measure ALLO in saliva. We validated two ELISAs, developed for use with blood, with the saliva samples of 25 pregnant women, examining the range and sensitivity, intra- and inter-assay precision, parallelism, linearity of dilution, and recovery. The samples were simultaneously analysed using the liquid-chromatography–mass-spectrometry (LC-MS) method. The kits differed in range (31.2–2000 pg/mL vs. 1.6–100 ng/mL) and sensitivity (<9.5 pg/mL vs. 0.9 ng/mL), with the latter showing significant matrix effects and the former fulfilling the acceptance criteria of all the parameters. The concentrations measured with LC–MS were below the lower limit of quantification (<1.0 ng/mL) and no signal was detected. One of the tested ELISAs is a valid method for detecting ALLO in the saliva of pregnant women. It has a suitable measurement range and higher sensitivity than the conventional LC–MS method.
Keywords
ELISA, Salivary bioscience, Allopregnanolone, Reproductive mood disorders, Pregnancy
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ISBN
ISSN
2218-273X
Language
English
Created during FHNW affiliation
Yes
Strategic action fields FHNW
Publication status
Published
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Peer review of the complete publication
Open access category
Gold
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Citation
Grötsch, M. K., Wietor, D. M., Hettich, T., & Ehlert, U. (2022). Validation of a commercial enzyme-linked immunosorbent assay for allopregnanolone in the saliva of healthy pregnant women. Biomolecules, 12(10), 1–10. https://doi.org/10.3390/biom12101381