Exploring the potential of various cyclodextrin‐based derivatives in enzyme supramolecular engineering
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Publikationsdatum
28.11.2024
Typ der Arbeit
Studiengang
Typ
01A - Beitrag in wissenschaftlicher Zeitschrift
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Herausgeber:in (Körperschaft)
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Übergeordnetes Werk
ChemBioChem
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Wiley
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Zusammenfassung
Enzyme stability and activity are pivotal factors for their implementation in different industrial applications. Enzyme supramolecular engineering relies on the fabrication of a tailor-made enzyme nano-environment to ensure enzyme stability without impairing activity. Cyclodextrins (CDs), cyclic oligomers of glucose, act as protein chaperones and stabilize, upon interaction with hydrophobic amino acid residues exposed at the protein surface, its three-dimensional structure. When used to build an organosilica layer shielding an enzyme, they enhance the protective effect of this layer. In the present study, we systematically assessed the protective effects of three organosilane derivatives based on ɑ-, β- and γ-CDs. A model lipase enzyme was immobilized at the surface of silica nanoparticles and shielded in an organosilica layer containing these organosilanes. Besides layer thickness optimization, the effect of different stressors (i. e., temperature, SDS, urea) was tested. Our results showed that organosilica layers produced with CDs improve enzyme thermal stability. They also support enzyme refolding after denaturation under chaotic conditions. Additionally, we demonstrated that the protective effect of the smallest CD derivative tested, namely ɑ-CD, surpassed the other macrocycles studied for conferring the immobilized enzyme with higher resistance to stress conditions. This protection strategy was also applied to a thermostable β-galactosidase enzyme.
Schlagwörter
Fachgebiet (DDC)
500 - Naturwissenschaften und Mathematik
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ISBN
ISSN
1439-4227
1439-7633
1439-7633
Sprache
Englisch
Während FHNW Zugehörigkeit erstellt
Ja
Zukunftsfelder FHNW
Publikationsstatus
Veröffentlicht
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Peer-Review der ganzen Publikation
Open Access-Status
Closed
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Zitation
FOROUTAN KALOURAZI, Ali, Amir NAZEMI, Ajmal UNNIRAM, Manuel FERRER, S. Shirin SHAHANGIAN und Patrick SHAHGALDIAN, 2024. Exploring the potential of various cyclodextrin‐based derivatives in enzyme supramolecular engineering. ChemBioChem. 28 November 2024. DOI 10.1002/cbic.202400840. Verfügbar unter: https://irf.fhnw.ch/handle/11654/49943