Development and full validation of an UPLC-MS/MS method for the quantification of the plant-derived alkaloid indirubin in rat plasma
dc.accessRights | Anonymous | |
dc.audience | Science | |
dc.contributor.author | Jähne, Evelyn A. | |
dc.contributor.author | Butterweck, Veronika | |
dc.contributor.author | Hamburger, Matthias | |
dc.contributor.author | Oufir, Mouhssin | |
dc.contributor.author | Sampath, Chethan | |
dc.date.accessioned | 2016-11-09T11:29:42Z | |
dc.date.available | 2016-11-09T11:29:42Z | |
dc.date.issued | 2016 | |
dc.description.abstract | An UPLC-MS/MS method for the quantification of indirubin in lithium heparinized rat plasma was developed and validated according to current international guidelines. Indirubin was extracted from rat plasma by using Waters Ostro™ pass-through sample preparation plates. The method was validated with a LLOQ of 5.00ng/mL and an ULOQ of 500ng/mL. The calibration curve was fitted by least-square quadratic regression, and a weighting factor of 1/X was applied. Recoveries of indirubin and I.S. were consistent and ≥75.5%. Stability studies demonstrated that indirubin was stable in lithium heparinized rat plasma for at least 3 freeze/thaw cycles, for 3h at RT, for 96h in the autosampler at 10°C, and for 84days when stored below -65°C. Preliminary pharmacokinetic (PK) data were obtained from Sprague Dawley rats after intravenous administration of indirubin (2mg/kg b.w.) and blood sampling up to 12h after injection. PK parameters were determined by non-compartmental analysis. Indirubin had a half-life (t1/2) of 35min, and a relatively high clearance (CL) of 2.71L/h/kg. | |
dc.identifier.doi | 10.1016/j.jpba.2016.05.018 | |
dc.identifier.issn | 0731-7085 | |
dc.identifier.issn | 1873-264X | |
dc.identifier.uri | http://hdl.handle.net/11654/23428 | |
dc.language.iso | en | |
dc.publisher | Elsevier | en_US |
dc.relation.ispartof | Journal of Pharmaceutical and Biomedical Analysis | en_US |
dc.title | Development and full validation of an UPLC-MS/MS method for the quantification of the plant-derived alkaloid indirubin in rat plasma | |
dc.type | 01A - Beitrag in wissenschaftlicher Zeitschrift | |
dc.volume | 128 | |
dspace.entity.type | Publication | |
fhnw.InventedHere | Yes | |
fhnw.IsStudentsWork | no | |
fhnw.PublishedSwitzerland | No | |
fhnw.ReviewType | Anonymous ex ante peer review of a complete publication | |
fhnw.affiliation.hochschule | Hochschule für Life Sciences | de_CH |
fhnw.affiliation.institut | Institut für Pharma Technology | de_CH |
fhnw.pagination | 247-252 | |
fhnw.publicationOnline | Ja | |
fhnw.publicationState | Published |
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