Biomimetic modification of titanium dental implant model surfaces using the RGDSP-peptide sequence. A cell morphology study
Vorschaubild nicht verfügbar
Autor:innen
Schuler, Martin
Owen, Gethin Rh.
Hamilton, Douglas W.
Textor, Marcus
Brunette, Donald M.
Tosatti, Samuele G.P.
Autor:in (Körperschaft)
Publikationsdatum
07/2006
Typ der Arbeit
Studiengang
Typ
01A - Beitrag in wissenschaftlicher Zeitschrift
Herausgeber:innen
Herausgeber:in (Körperschaft)
Betreuer:in
Übergeordnetes Werk
Biomaterials
Themenheft
DOI der Originalpublikation
Link
Reihe / Serie
Reihennummer
Jahrgang / Band
27
Ausgabe / Nummer
21
Seiten / Dauer
4003-4015
Patentnummer
Verlag / Herausgebende Institution
Elsevier
Verlagsort / Veranstaltungsort
Auflage
Version
Programmiersprache
Abtretungsempfänger:in
Praxispartner:in/Auftraggeber:in
Zusammenfassung
Surface topography and (bio)chemistry are key factors in determining cell response to an implant. We investigated cell adhesion and spreading patterns of epithelial cells, fibroblasts and osteoblasts on biomimetically modified, smooth and rough titanium surfaces. The RGD bioactive peptide sequence was immobilized via a non-fouling poly(l-lysine)-graft-poly(ethylene glycol) (PLL-g-PEG) molecular assembly system, which allowed exploitation of specific cell–peptide interactions even in the presence of serum. As control surfaces, bare titanium and bio-inactive surfaces (scrambled RDG and unfunctionalized PLL-g-PEG) were used. Our findings demonstrated that surface topography and chemistry directly influenced the attachment and morphology of all cell types tested. In general, an increase in cell number and more spread cells were observed on bioactive substrates (containing RGD) compared to bio-inactive surfaces. More fibroblasts were present on smooth than on rough topographies, whereas for osteoblasts the opposite tendency was observed. Epithelial cell attachment did not follow any regular pattern. Footprint areas for all cell types were significantly reduced on rough compared to smooth surfaces. Osteoblast attachment and footprint areas increased with increasing RGD-peptide surface density. However, no synergy (interaction) between RGD-peptide surface density and surface topography was observed for osteoblasts neither in terms of attachment nor footprint area.
Schlagwörter
Titanium oxide, Surface topography, Surface modification, Biomimetic material, Peptide, Cell morphology
Fachgebiet (DDC)
600 - Technik, Medizin, angewandte Wissenschaften
Veranstaltung
Startdatum der Ausstellung
Enddatum der Ausstellung
Startdatum der Konferenz
Enddatum der Konferenz
Datum der letzten Prüfung
ISBN
ISSN
0142-9612
Sprache
Englisch
Während FHNW Zugehörigkeit erstellt
Nein
Publikationsstatus
Veröffentlicht
Begutachtung
Peer-Review der ganzen Publikation
Open Access-Status
Closed
Lizenz
Zitation
SCHULER, Martin, Gethin Rh. OWEN, Douglas W. HAMILTON, Michael DE WILD, Marcus TEXTOR, Donald M. BRUNETTE und Samuele G.P. TOSATTI, 2006. Biomimetic modification of titanium dental implant model surfaces using the RGDSP-peptide sequence. A cell morphology study. Biomaterials. Juli 2006. Bd. 27, Nr. 21, S. 4003–4015. DOI 10.1016/j.biomaterials.2006.03.009. Verfügbar unter: https://irf.fhnw.ch/handle/11654/45804