Biomimetic modification of titanium dental implant model surfaces using the RGDSP-peptide sequence. A cell morphology study
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Author (Corporation)
Publication date
07.2006
Typ of student thesis
Course of study
Type
01A - Journal article
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Parent work
Biomaterials
Special issue
DOI of the original publication
Link
Series
Series number
Volume
27
Issue / Number
21
Pages / Duration
4003-4015
Patent number
Publisher / Publishing institution
Elsevier
Place of publication / Event location
Edition
Version
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Practice partner / Client
Abstract
Surface topography and (bio)chemistry are key factors in determining cell response to an implant. We investigated cell adhesion and spreading patterns of epithelial cells, fibroblasts and osteoblasts on biomimetically modified, smooth and rough titanium surfaces. The RGD bioactive peptide sequence was immobilized via a non-fouling poly(l-lysine)-graft-poly(ethylene glycol) (PLL-g-PEG) molecular assembly system, which allowed exploitation of specific cell–peptide interactions even in the presence of serum. As control surfaces, bare titanium and bio-inactive surfaces (scrambled RDG and unfunctionalized PLL-g-PEG) were used. Our findings demonstrated that surface topography and chemistry directly influenced the attachment and morphology of all cell types tested. In general, an increase in cell number and more spread cells were observed on bioactive substrates (containing RGD) compared to bio-inactive surfaces. More fibroblasts were present on smooth than on rough topographies, whereas for osteoblasts the opposite tendency was observed. Epithelial cell attachment did not follow any regular pattern. Footprint areas for all cell types were significantly reduced on rough compared to smooth surfaces. Osteoblast attachment and footprint areas increased with increasing RGD-peptide surface density. However, no synergy (interaction) between RGD-peptide surface density and surface topography was observed for osteoblasts neither in terms of attachment nor footprint area.
Keywords
Titanium oxide, Surface topography, Surface modification, Biomimetic material, Peptide, Cell morphology
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ISBN
ISSN
0142-9612
Language
English
Created during FHNW affiliation
No
Strategic action fields FHNW
Publication status
Published
Review
Peer review of the complete publication
Open access category
Closed
License
Citation
Schuler, M., Owen, G. Rh., Hamilton, D. W., de Wild, M., Textor, M., Brunette, D. M., & Tosatti, S. G. P. (2006). Biomimetic modification of titanium dental implant model surfaces using the RGDSP-peptide sequence. A cell morphology study. Biomaterials, 27(21), 4003–4015. https://doi.org/10.1016/j.biomaterials.2006.03.009