Production of superparamagnetic nanobiocatalysts for green chemistry applications
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Publikationsdatum
23.04.2016
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Studiengang
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01A - Beitrag in wissenschaftlicher Zeitschrift
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Übergeordnetes Werk
Applied Microbiology and Biotechnology
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Zusammenfassung
Immobilization of enzymes on solid supports is a convenient method for increasing enzymatic stability and enabling enzyme reuse. In the present work, a sorption-assisted surface conjugation method was developed and optimized to immobilize enzymes on the surface of superparamagnetic nanoparticles. An oxidative enzyme, i.e., laccase from Trametes versicolor was used as model enzyme. The immobilization method consists of the production of superparamagnetic nanoparticles by co-precipitation of FeCl2 and FeCl3. Subsequently, the particle surface is modified with an organosilane containing an amino group. Next, the enzymes are adsorbed on the particle surface before a cross-linking agent, i.e., glutaraldehyde is added which links the amino groups on the particle surface with the amino groups of the enzymes and leads to internal cross-linking of the enzymes as well. The method was optimized using response surface methodology regarding optimal enzyme and glutaraldehyde amounts, pH, and reaction times. Results allowed formulation of biocatalysts having high specific enzymatic activity and improved stability. The biocatalysts showed considerably higher stability compared with the dissolved enzymes over a pH range from 3 to 9 and in the presence of several chemical denaturants. To demonstrate the reusability of the immobilized enzymes, they were applied as catalysts for the production of a phenoxazinone dye. Virtually, 100 % of the precursor was transformed to the dye in each of the ten conducted reaction cycles while on average 84.5 % of the enzymatic activity present at the beginning of a reaction cycle was retained after each cycle highlighting the considerable potential of superparamagnetic biocatalysts for application in industrial processes.
Schlagwörter
Immobilization, Laccase, Magnetite nanoparticles, Phenoxazinone dye, Magnetic biocatalysts
Fachgebiet (DDC)
500 - Naturwissenschaften
570 - Biowissenschaften, Biologie
570 - Biowissenschaften, Biologie
Veranstaltung
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ISBN
ISSN
1432-0614
0175-7598
0175-7598
Sprache
Englisch
Während FHNW Zugehörigkeit erstellt
Ja
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Publikationsstatus
Veröffentlicht
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Peer-Review der ganzen Publikation
Open Access-Status
Lizenz
Zitation
GASSER, Christoph, Erik AMMANN, Andreas SCHÄFFER, Patrick SHAHGALDIAN und Philippe CORVINI, 2016. Production of superparamagnetic nanobiocatalysts for green chemistry applications. Applied Microbiology and Biotechnology. 23 April 2016. DOI 10.1007/s00253-016-7479-7. Verfügbar unter: http://hdl.handle.net/11654/17942