In vitro modulation of inflammatory target gene expression by a polyphenol-enriched fraction of rose oil distillation waste water

dc.accessRightsAnonymous
dc.audienceScience
dc.contributor.authorWeston, Anna
dc.contributor.authorRausenberger, Julia
dc.contributor.authorButterweck, Veronika
dc.contributor.authorWedler, Jonas
dc.date.accessioned2016-12-16T11:39:58Z
dc.date.available2016-12-16T11:39:58Z
dc.date.issued2016
dc.description.abstractClassical production of rose oil is based on water steam distillation from the flowers of Rosa damascena. During this process, large quantities of waste water accrue which are discharged to the environment, causing severe pollution of both, groundwater and surface water due to a high content of polyphenols. We recently developed a strategy to purify the waste water into a polyphenol-depleted and a polyphenol-enriched fraction RF20-(SP-207). RF20-(SP-207) and sub-fraction F(IV) significantly inhibited cell proliferation and migration of HaCaT cells. Since there is a close interplay between these actions and inflammatory processes, here we focused on the fractions' influence on pro-inflammatory biomarkers. HaCaT keratinocytes were treated with RF20-(SP-207), F(IV) (both at 50 μg/mL) and ellagic acid (10 μM) for 24 h under TNF-α (20 ng/mL) stimulated and non-stimulated conditions. Gene expression of IL-1β, IL-6, IL-8, RANTES and MCP-1 was analyzed by reverse transcriptase polymerase chain reaction (RT-PCR) and cellular protein secretion of IL-8, RANTES and MCP-1 was determined by ELISA based assays. RF20-(SP-207) and F(IV) significantly decreased the expression and cellular protein secretion of IL-1β, IL-6, IL-8, RANTES and MCP-1. The diminishing effects on inflammatory target gene expression were slightly less pronounced under TNF-α stimulated conditions. In conclusion, the recovered polyphenol fraction RF20-(SP-207) from rose oil distillation waste water markedly modified inflammatory target gene expression in vitro, and, therefore, could be further developed as alternative treatment of acute and chronic inflammation.
dc.identifier.doihttp://dx.doi.org/10.1016/j.fitote.2016.08.019
dc.identifier.issn1873-6971
dc.identifier.issn0367-326X
dc.identifier.urihttp://hdl.handle.net/11654/23746
dc.language.isoen
dc.publisherElsevieren_US
dc.relation.ispartofFitoterapiaen_US
dc.subjectAnti-inflammatory
dc.subjectELISA
dc.subjectRT-PCR
dc.subjectRosa damascena
dc.subjectWaste water
dc.titleIn vitro modulation of inflammatory target gene expression by a polyphenol-enriched fraction of rose oil distillation waste water
dc.type01A - Beitrag in wissenschaftlicher Zeitschrift
dc.volume114
dspace.entity.typePublication
fhnw.InventedHereYes
fhnw.IsStudentsWorkno
fhnw.PublishedSwitzerlandNo
fhnw.ReviewTypeAnonymous ex ante peer review of a complete publication
fhnw.affiliation.hochschuleHochschule für Life Sciences FHNWde_CH
fhnw.affiliation.institutInstitut für Pharma Technologyde_CH
fhnw.pagination56-62
fhnw.publicationOnlineJa
fhnw.publicationStatePublished
relation.isAuthorOfPublication75b72169-64d8-4e5c-9fce-ffe0de0b00fc
relation.isAuthorOfPublicationddc3ca4a-f9b2-4770-882c-c7b7b85d222d
relation.isAuthorOfPublication.latestForDiscovery75b72169-64d8-4e5c-9fce-ffe0de0b00fc
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