Extended and Fully Automated Newborn Screening Method for Mass Spectrometry Detection
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Publication date
12/2017
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01A - Journal article
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International Journal of Neonatal Screening
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4
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1
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MDPI
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Abstract
A new and fully automated newborn screening method for mass spectrometry was introduced in this paper. Pathological relevant amino acids, acylcarnitines, and certain steroids are detected within 4 min per sample. Each sample is treated in an automated and standardized workflow, where a mixture of deuterated internal standards is sprayed onto the sample before extraction. All compounds showed good linearity, and intra- and inter-day variation lies within the acceptance criteria (except for aspartic acid). The described workflow decreases analysis cost and labor while improving the sample traceability towards good laboratory practice.
Keywords
newborn screening (NBS), dried blood spots (DBS), automation
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2409-515X
Language
English
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Yes
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Published
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Citation
GAUGLER, Stephan, Jana RYKL, Irene WAGNER, Tamara VON DÄNIKEN, Ralph FINGERHUT und Götz SCHLOTTERBECK, 2017. Extended and Fully Automated Newborn Screening Method for Mass Spectrometry Detection. International Journal of Neonatal Screening. Dezember 2017. Bd. 4, Nr. 1. DOI 10.3390/ijns4010002. Verfügbar unter: http://hdl.handle.net/11654/26974